Kaito Sugimoto, Christos S. Karamitros, Jun-ichi Horiuchi, Yoichi Kumada. Identification and characterization of rabbit scFv antibodies suitable for immuno-affinity separation of recombinant human kynureninase from Escherichia coli cell lysate. Journal of Bioscience and Bioengineering. 2024
Sofia De Felice, Zhanna Romanyuk, Monica Chinellato, Giulia Zoia, Sara Linciano, Yoichi Kumada, Els Pardon, Jan Steyaert, Alessandro Angelini, Laura Cendron. Crystal structure of human serum albumin in complex with megabody reveals unique human and murine cross-reactive binding site. Protein science : a publication of the Protein Society. 2024. 33. 2. e4887
Yoichi Kumada, Reina Tanibata, Kimitaka Yamamoto, Hiroshi Noguchi, Alessandro Angelini, Jun-Ichi Horiuchi. Development and characterization of a latex turbidimetric immunoassay using rabbit anti-CRP single-chain Fv antibodies. Journal of immunological methods. 2023. 520. 113522-113522
John Blazeck, Christos S. Karamitros, Kyle Ford, Catrina Somody, Ahlam Qerqez, Kyle Murray, Nathaniel T. Burkholder, Nicholas Marshall, Anirudh Sivakumar, Wei-Cheng Lu, et al. Bypassing evolutionary dead ends and switching the rate-limiting step of a human immunotherapeutic enzyme. Nature Catalysis. 2022. 5. 10. 952-967
Yoichi Kumada, Haingomaholy Michelle Rakotondravao, Yuya Hasegawa, Yuki Iwashita, Hiromichi Okura, Seiichi Uchimura, Jun-ichi Horiuchi. Strategies for selection and identification of rabbit single-chain Fv antibodies as ligand in affinity chromatography. Journal of Bioscience and Bioengineering. 2022. 134. 3. 233-239
Frame-switching technology for generation of rabbit single-chain Fv antibodies with different physicochemical properties.
(38th Annual Symposium (PS38), Protein Society 2024)
Development of Rabbit ScFv-Based Immunoaffinity Chromatography for Purification of Human Kynureninase
(PREP 2024 2024)
Design and Site-directed immobilization of recombinant antibody fragments genetically-fused with materials-binding peptides for sensitive immuno-sensing
(Bio-sensing 2024 2024)
Design and site-directed immobilization of recombinant antibody fragments for sensitive immuno-diagnostic
(GMBB2024 2024)
Potentials of rabbit scFv antibodies as a ligand for on-demand affinity purification of non-antibody biotherapeutics
(Peptalk2024 2024)