Takao Toshifumi

タカオトシフミ | Takao Toshifumi

Affiliation and department：
Job title： Specially Appointed Professor

Research field (1)： Structural biochemistry

Research theme for competitive and other funds (30)：

2022 - 2025 尿中蛋白質断片の網羅的解析による日和見感染症の新規診断法の開発
2017 - 2022 Molecular dissection of robust and flexible circadian clock and its control animal physiology
2014 - 2017 Regulation of membrane traffic of Wnt and its receptors
2013 - 2017 Studies on the physiological role of Prx4 in oxidative protein folding
2011 - 2015 Amino acid sequence analysis of fossil proteins using mass spectrometry

2011 - 2014 Development of tissue peptidome analysis technology and its application to discovery of new biologically active peptides

2011 - 2013 Selective activation of Wnt signaling pathway using with purified Wnt proteins

2010 - 2012 Mass Spectrometry of Metalloproteins and High-Molecular-Weight Protein Complexes

2010 - 2011 A novel drug carrier based on the collagen-like triple-helical peptide

2006 - 2010 Structural basis of functional coupling between transcription and cellular metabolism

2007 - 2008 Diagnostic system for acute cellular rejection in livertransplantation using protemomic analysis of bile

2003 - 2007 Molecular machinery regulating energy and metabolic networks in plant cells

2003 - 2005 植物細胞エネルギー・代謝ネットワークを制御する分子マシナリー

2003 - 2004 質量分析法によるリガンド-レセプター相互作用の精密解析

2002 - 2003 Dynamism analysis of posttranslational modifications by mass spectrometry.

2000 - 2001 Development of the method for high-sensitivity structural analysis of carbohydrate moieties of a glycoprotein by mass spectrometry

2000 - 2000 プロテオーム研究の国際的動向と将来展望に関する調査研究

1998 - 1999 Development of a high-energy collision-induced dissociation device for high-sensitivity and high-resolution MS/MS/MS

1996 - 1997 Development of a Full-Automatic Equipment for Gas-Phase Chemical Reaction Intended for Microanalysis of Protein Primary Structures

1994 - 1995 Capillary HPLC/electrospray ionization mass spectrometry for structural analysis of micro-quantities of proteins

1993 - 1993 質量分析による糖蛋白質糖鎖構造の解析法に関する研究

1992 - 1993 A New Pulsed FAB Control System Coupled with an Array Detector on a Four-Sector Tandem Mass Spectrometer

1990 - 1991 A Control System for a Four-Sector Tandem Mass Spectrometer

1988 - 1988 質量合析による遺伝子組換え蛋白質の構造解析法に関する研究

プロテオミクス関連装置の開発

高感度・高出力質量分析計の開発

質量分析による蛋白質の構造解析

Development of proteomic apparatus

Development of high-sensitivity and high-throughput mass spectrometer

Structural Analysis of Proteins by Mass Spectrometry

Show all

Papers (115)：

Rika Inomata, Hironobu Tsubouchi, Toshifumi Takao, Mone Kurokawa, Shigehisa Yanagi, Katsuya Sakai, Taiga Miyazaki. Resolvin D4 mitigates lipopolysaccharide-induced lung injury in mice. Prostaglandins, Leukotrienes and Essential Fatty Acids. 2024. Volume 203,
Satomy Pousa a, Pablo E. Ramos-Bermúdez b, Vladimir Besada a, Paulo Carvalho c, Louise Kur, Ulrich c, Michel Batista c, Rodrigo Soares Caldeira Brant c, Olivia Martínez d, Alejandro Leyva e, et al. On the utility of the extracted ion chromatograms for assigning the conjugation sites and side reactions in bioconjugates synthesized by the maleimide-thiol chemistry. Microchemical Journal. 2024
Pablo E. Ramos-Bermúdez, Satomy Pousa, Paulo Carvalho, Rodrigo Soares, Caldeira Bran, Michel Batista, Hironobu Hojo, Hilda E. Garay, Abel Roscoe, Alina Rodríguez Mallón, et al. A hydrolyzed N-propionylthiosuccinimide linker is cleaved by metastable fragmentation, increasing reliability of conjugation site identification in conjugate vaccines. Rapid Communications in Mass Spectrometry:. 2024
Shuichi Mitsunaga, Nobuaki Okumura, Toshiki Takei, Toshifumi Takao, Hironobu Tsubouchi, Kohei Nakata, Masafumi Nakamura, Yuji Kitahata, Hideki Motobayashi, Masafumi Ikeda, et al. Identification of a urinary CD276 fragment for detecting resectable pancreatic cancer using a C-terminal proteomics strategy. Scientific Reports. 2024. 14. 1
Yue Pan, Qiuyi Wang, Mengyao Chen, Toshifumi Takao. Profiling of urinary steroids aided by lithium ion adduction-based ultrahigh-performance liquid chromatography-tandem mass spectrometry. Rapid Communications in Mass Spectrometry. 2024

ｍore...

MISC (170)：

HOJO Hironobu, TAKEI Toshiki, ASAHINA Yuya, OKUMURA Nobuaki, TAKAO Toshifumi, SO Masatomo, SUETAKE Isao, SATO Takeshi, KAWAMOTO Akihiro, HIRABAYASHI Yoshio. CHEMICAL SYNTHESIS OF MEMBRANE PROTEIN CAVEOLIN AND ITS MEMBRANE INSERTION. ペプチド討論会講演要旨集. 2020. 57th
清水貴美子, 前畑佳納子, 池野知子, Qiuyi Wang, 高尾敏文, 深田吉孝. 行動を制御する脳内分子メカニズムとそのダイナミクス記憶の概日変動を生み出す分子制御メカニズム. 日本生化学会大会プログラム・講演要旨集. 2019. 92回. [3S11m-01]
大野泉, 光永修一, 菅元康, 池田公史, 坪内拡伸, 柳重久, 奥村宜明, 高尾敏文, 中里雅光. 新たな早期膵癌診断マーカーとしての尿中キニノーゲン(KNG)断片の可能性. 膵臓(Web). 2019. 34. 3. A260-A261(J-STAGE)-A261
N. Itai, R. Okabe, T. Shimazu, T. Takao, Y. Kaburagi, T. Dohi, T. Tonozuka, A. Nishikawa. Phosphorylation of sorting nexin 5 regulates vesicular trafficking. MOLECULAR BIOLOGY OF THE CELL. 2015. 26
貝森淳哉, 前原一満, 畑中雅喜, 小尾佳嗣, 津田秀年, 山本聡子, 市丸直嗣, 楽木宏実, 高原史郎, 大川恭行, et al. 抑制性ヒストンアセチル化修飾H4K20acはGcn5によって制御され、臓器肥大により誘導される. 日本腎臓学会誌. 2014. 56. 3. 415-415

ｍore...

Books (15)：

蛋白質・核酸・酵素"蛋白質の質量分析(1)磁場型質量分析装置とその応用"
共立出版 2000
プロテオーム解析法(実験医学別冊)"De novo sequencingのための解析ソフトウェア"
羊土社 2000
Mass spectrometry of a protein(1) : Magnetic sector mass spectrometer and its application
2000
Software aid for de novo sequencing
2000
質量分析計による分子量の精密測定。
秀潤社 1998

ｍore...

Lectures and oral presentations (1)：

Active ternary complex of ribonuclease HI: RNA/DNA hybrid: metal ions probed by ESI mass
(2022)

Works (5)：

植物細胞エネルギー・代謝ネットワークを制御する分子マシナリー
2004 -
質量分析法によるイネ組織・時期特異的超微量タンパク質の解析及び翻訳後修飾情報(糖タンパク質)の構造解析
2000 -
Proteomic analysis of rice proteins and their post-translational modifications(such as glycosylation)in different tissues or under different conditions by mass spectrometry
2000 -
質量分析法による生体内ペプチドの超微量構造解析に関する研究
1999 -
Research on structural analysis of biological peptides by mass spectrometry
1999 -

Education (4)：

- 1986 Osaka University
- 1986 Osaka University Graduate School, Division of Natural Science
- 1981 Osaka University School of Science
- 1981 Osaka University Faculty of Science

Professional career (1)：

(BLANK) (Osaka University)

Work history (5)：

2024/05 - 現在 Osaka University Institute for Protein Research Specially Appointed Professor
1987 - 1996 Osaka University Institute for Protein Research
1987 - 1996 Osaka University, Institute for Protein Research, Instructor
1996 - - 大阪大学蛋白質研究所助教授
1996 - - Osaka University, Institute for Protein Research, Associate Professor

Awards (3)：

2022/11 - from the University of Havana Dr. Honoris Causa
1994 - 日本生化学会奨励賞
1992 - 内藤記念科学奨励金

Association Membership(s) (5)：

日本質量分析学会 , 日本ペプチド学会 , アメリカ質量分析学会(The American Society for Mass Spectrometry) , 日本生化学会 , 日本化学会

※ Researcher’s information displayed in J-GLOBAL is based on the information registered in researchmap. For details, see here.

Return to Previous Page