2017 - 2020 Synthesis of nitrogen-containing useful compounds with the enzymes modified based on their reaction mechanisms
2016 - 2018 Development of novel microbial oxidation-reduction/electron transferring function acting under high temperature and high alkaline conditions
2010 - 2011 Design of novel branched chain amino acids with metabolic control function for clinical improvement of lifestyle diseases.
2007 - 2008 非侵襲的細胞脂質解析を目指した生体触媒モジュールの開発
2006 - 2008 Production of novel functional lipids by manifold application of microbial capacity
2006 - 2007 Microbial production process for stereospecific synthesis of nitrogen-containing chiral compounds
Show all
Papers (51)：
Chee-Leong Soong, Kengo Deguchi, Michiki Takeuchi, Syoko Kozono, Nobuyuki Horinouchi, Dayong Si, Makoto Hibi, Sakayu Shimizu, Jun Ogawa. Gene identification and enzymatic characterization of the initial enzyme in pyrimidine oxidative metabolism, uracil-thymine dehydrogenase. Journal of Bioscience and Bioengineering. 2024
Taku Mizutani, Ryotaro Hara, Michiki Takeuchi, Makoto Hibi, Makoto Ueda, Jun Ogawa. One-Pot Synthesis of Useful S-Substituted-l-cysteine Sulfoxides Using Genetically Engineered Escherichia coli. Journal of Agricultural and Food Chemistry. 2024. 72. 10. 5339-5347
Samantha DM Arras, Nellie SibaevaRyan, J Catchpole, Nobuyuki Horinouchi, Dayong Si, Alannah M Rickerby, Kengo Deguchi, Makoto Hibi, Koichi Tanaka, Michiki Takeuchi, et al. Characterisation of an Escherichia coli line that completely lacks ribonucleotide reduction yields insights into the evolution of parasitism and endosymbiosis. eLife. 2023. e83845
Taku Mizutani, Ryotaro Hara, Takayuki Iihoshi, Shoko Kozono, Michiki Takeuchi, Makoto Hibi, Satomi Takahashi, Makoto Ueda, Jun Ogawa. Identification of tryptophanase from Escherichia coli for the synthesis of S-allyl-l-cysteine and related S-substituted cysteine derivatives. Journal of Bioscience and Bioengineering. 2022
Taku Mizutani, Ryotaro Hara, Michiki Takeuchi, Kazuo Yamagishi, Yoshinori Hirao, Kenichi Mori, Makoto Hibi, Makoto Ueda, Jun Ogawa. l-Tryptophan-starved cultivation enhances S-allyl-l-cysteine synthesis in various food-related microorganisms. Bioscience, biotechnology, and biochemistry. 2022. 86. 6. 792-799
出口賢児, SI Dayong, 堀之内伸行, 小園祥子, 竹内道樹, 日比慎, 日比慎, POOLE Anthony M., 小川順. Growth evaluation of Escherichia coli with knockout of deoxyribonucleotide synthetic pathway genes. 日本農芸化学会大会講演要旨集(Web). 2021. 2021
日比慎, 小川順. Screening and development of hydroxylation reactions creating amino acid diversity. 生化学. 2021. 93. 3
Green Biocatalysis
John Wiley & Sons, Inc. 2016 ISBN:9781118822296
Lectures and oral presentations (117)：
Elucidation of α-aminoisobutyric acid hydroxylase, a novel non-heme diiron enzyme from Rhodococcus wratislaviensis
(2nd Japan-Switzerland-Germany Workshop on Biocatalysis and Bioprocess Development 2021)
Characterization of uracil-thymine dehydrogenase in pyrimidine oxidative metabolism of Rhodococcus erythropolis JCM 3132
(1st Japan-Germany-Switzerland Workshop for Enzyme Technology and Bioprocess Development 2019)
Enzymatic process for useful amino acid synthesis using microbial Fe(II)/α-ketoglutarate-dependent dioxygenases
(1st Japan-Germany-Switzerland Workshop for Enzyme Technology and Bioprocess Development 2019)
Tandem enzymatic reactions for the production of a phytochemical L-(+)-alliin
(1st Japan-Germany-Switzerland Workshop for Enzyme Technology and Bioprocess Development 2019)
Production of (+)-alliin by tandem enzymatic reactions
(The 14th Asian Congress on Biotechnology 2019)
