HAYASHI Akiyo

ハヤシアキヨ | HAYASHI Akiyo

Affiliation and department：
Job title：助教

Research field (4)： Functional biochemistry , Cell biology , Genetics , Molecular biology

Research keywords (5)： DNA repair , PCNA , Cdt2 , DNA replication , Cell Cycle

Research theme for competitive and other funds (7)：

2023 - 2024 正確なDNA複製を保証するタンパク質分解系の分子構造基盤の解明
2020 - 2021 ゲノム維持に関わるCRL4-Cdt2ユビキチンリガーゼの活性制御機構の解析
2018 - 2021 Activation mechanism of CRL4-Cdt2 ubiquitin ligase after DNA damage
2018 - 2019 Cell Responses accompanied by DNA re-replication
2014 - 2018 Regulation of DNA replication through proteolysis coupled with PCNA cycle

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Papers (10)：

Mazian M, Suenaga N, Ishii T, Hayashi A, Shiomi Y, Nishitani H. A DNA-binding domain in the C-terminal region of Cdt2 enhances the DNA synthesis-coupled CRL4Cdt2 ubiquitin ligase activity for Cdt1. Journal of biochemistry. 2019. 165. 6. 505-516
Akiyo Hayashi, Nickolaos Nikiforos Giakoumakis, Tatjana Heidebrecht, Takashi Ishii, Andreas Panagopoulos, Christophe Caillat, Michiyo Takahara, Richard G Hibbert, Naohiro Suenaga, Magda Stadnik-Spiewak, et al. Direct binding of Cdt2 to PCNA is important for targeting the CRL4Cdt2 E3 ligase activity to Cdt1. Life Science Alliance. 2018. 1. 6. e201800238-e201800238
Akiyo Hayashi, Nickolaos Nikiforos Giakoumakis, Tatjana Heidebrecht, Takashi Ishii, Andreas Panagopoulos, Christophe Caillat, Michiyo Takahara, Richard G Hibbert, Naohiro Suenaga, Magda Stadnik-Spiewak, et al. Direct binding of Cdt2 to PCNA is important for targeting the CRL4Cdt2 E3 ligase activity to Cdt1. bioRxiv. 2018
Kohei Nukina, Akiyo Hayashi, Yasushi Shiomi, Kaoru Sugasawa, Motoaki Ohtsubo, Hideo Nishitani. Mutations at multiple CDK phosphorylation consensus sites on Cdt2 increase the affinity of CRL4Cdt2 for PCNA and its ubiquitination activity in S phase. Genes to Cells. 2018. 23. 3. 200-213
Miyuki Tanaka, Michiyo Takahara, Kohei Nukina, Akiyo Hayashi, Wataru Sakai, Kaoru Sugasawa, Yasushi Shiomi, Hideo Nishitani. Mismatch repair proteins recruited to ultraviolet light-damaged sites lead to degradation of licensing factor Cdt1 in the G1 phase. CELL CYCLE. 2017. 16. 7. 673-684

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Lectures and oral presentations (22)：

The accumulation mechanism of CRL4-Cdt2 ubiquitin ligase at the DNA replication sites by live imaging analysis
(The 46th Annual Meeting of the Molecular Biology Society of Japan 2023)
Analysis of DNA re-replication initiation process induced by MLN4924
(The 46th Annual Meeting of the Molecular Biology Society of Japan 2023)
Analysis of Intracellular Ca2+ response in DNA Re-replicating Cells
(The 46th Annual Meeting of the Molecular Biology Society of Japan 2023)
DNA再複製に伴う細胞内Ca2+ 濃度変化の解析
(第45回日本分子生物学会年会 2022)
DNA損傷修復において機能するCRL4-Cdt2 ユビキチンリガーゼの損傷部位集積機構のライブイメージング解析
(第44回日本分子生物学会年会 2021)

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Education (2)：

2013 - 2016 University of Hyogo Graduate School of Life Science
2011 - 2013 University of Hyogo Graduate School of Life Science Master

Professional career (2)：

修士(理学) (兵庫県立大学)
博士(理学) (兵庫県立大学)

Work history (2)：

2016/04 - 現在 University of Hyogo Graduate School of Life Science Assistant professor
2013/04 - 2016/03 JSPS Research Fellowships for Young Scientists (Doctoral Course 1)

Awards (1)：

2013/11 - 第22回DNA複製・組換え・修復ワークショップ若手研究者優秀発表賞選考委員会若手研究者優秀発表賞口頭発表の部クロマチン上のPCNAに依存したCRL4Cdt2のユビキチン化機構

Association Membership(s) (1)：

THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

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