Research theme for competitive and other funds (16):
2020 - 2023 ゲノム編集システムを利用した高効率クエン酸生産糸状菌の育種
2013 - 2016 Generation of the cell factories for bio-based organic acid production through improvement of filamentous fungi by modification of transport system
2010 - 2013 Generation of the cell factories for bio-based organic acid production through improvement of filamentous fungi by metabolic engineering
1996 - 2004 Production of Menthol Glycosides by Microbial Enzymes
1993 - 2004 Biodesulfurization of Diesel Oil
1999 - 2003 微生物機能を利用した資源循環型水環境プロセスの構築
1994 - 2003 Ecology and Distribution of Microorganisms in the Long-Term Stockpiles of Crude Oil
1996 - 2000 生理活性素材開発
新規な生分解性界面活性物質の酵素法による合成
プロトプラスト融合法により作成した異属間雑種糸状菌のDNA構成と表現型
クエン酸生産糸状菌におけるオリゴマイシン耐性変異株のミトコンドリア遺伝子解析
Gene Cloning of the Enzymes Related to Citric Acid Production in Fungi
糸状菌由来のシアン非感受性呼吸系酵素遺伝子を利用した大腸菌の代謝機能改変
ダイナミックな分子デザインと変異集積による制限酵素の改変
Screening of a novel glucosyl condensing enzyme showing the activity of glycoside synthesis and the application of this enzyme to useful glycoside production
Biodesulfurization using thermophilic bacteria and identification of bds genes
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Papers (427):
Enzymatic Synthesis of Alkyl α-D-Glucopyranosides by XgtA, a Glucosyl Transfer Enzyme Derived from Xanthomonas campestris WU-9701. 2022
Evaluation of the mycotoxin non~production of Aspergillus tubingensis WU-223L, acitric acid hyperproducer, based on the genome and metabolites analysis. 2022
Yoshioka Isato, Kirimura Kohtaro. Rapid and marker-free gene replacement in citric acid-producing Aspergillus tubingensis (A. niger) WU-2223L by the CRISPR/Cas9 system-based genome editing technique using DNA fragments encoding sgRNAs. Journal of bioscience and bioengineering. 2021. 131. 6. 579-588
KOBAYASHI Keiichi, HONDA Yuki, KIRIMURA Kohtaro. 3P-1017 Disruption of ku80 in Citric Acid-Producing Aspergillus niger for Improvement of Homologous Recombination Frequencies. 2010. 22. 62-62
NOGUCHI Atsushi, NAKAZAWA Yuji, YAGASAKI Makoto, KIRIMURA Kohtaro, KINO Kuniki. 2C11-1 Synthesis of L-amino acid dipeptides using a novel L-amino acid ligase from Bacillus licheniformis. 2007. 19. 83-83
KATO Takayuki, FUKUSHIMA Naoki, KIRIMURA Kohtaro, KINO Kuniki. 1D12-1 Continuous Ethanol Fermentation by Saccharomyces cerevisiae X33. 2007. 19. 98-98
KURIHARA Ikumi, ISHII Yoshitaka, KIRIMURA Kohtaro, KINO Kuniki. 2D11-3 Cultivation Conditions for Production of Poly(arginyl-histidine) in Epichloe kibiensis E18. 2007. 19. 101-101
YONEYAMA Mariko, SATO Masaru, KIRIMURA Kohtaro, KINO Kuniki. 2F12-1 Site-Direct Mutagenesis on Ile^<384> and Tyr^<396> Residues of Broad Specificity Amino Acid Racemase from Pseudomonas putida IFO12996. 2006. 18. 102-102