Hayashi Hideyuki

ハヤシヒデユキ | Hayashi Hideyuki

Affiliation and department：
Job title： Professor

Research field (4)： Biochemistry , Biophysics , Functional biochemistry , Structural biochemistry

Research keywords (3)：蛋白質化学・生物物理化学 , Bipphysical Chemistry , Enzyme Chemistry

Research theme for competitive and other funds (28)：

2022 - 2025 Visualization of protonated states of dissociative substituents by cooperation of quantum calculations and crystal structures
2020 - 2023 非凍結結晶を用いた“in crystallo”酵素反応解析
2017 - 2021 In crystallo thermodynamic analysis of the cofactor conformational change of copper amine oxidase
2015 - 2019 Concerted proton/electron transfer mechanism in cofactor-dependent enzyme
2014 - 2018 X-ray and neutron crystallographic structure of copper amine oxidase

2014 - 2017 Theoretical elucidation on the full reaction mechanisms of threonine synthase

2012 - 2016 Reaction specificity of pyridoxal enzymes

2009 - 2011 Studies on the molecular mechanism of sphingolipid metabolism based on the crystal structure of the enzymes

2009 - 2011 Multidimensional energy analysis of pyridoxal-enzyme and quinoenzyme reactions

2006 - 2006 Theoretical analysis of enzyme catalysis

2006 - 2006 Kinetic analysis of enzyme catalysis

2004 - 2005 Studies on the proton transfer processes in pyridoxal enzymes

2004 - 2005 Mechanism of Enzyme Functions

2001 - 2003 Designing composite biocatalysts by analysis of the proton-transfer process in pyridoxal enzymes

2001 - 2003 Molecular Design and Evolution Engineering for Compositc Biochatalysts

2001 - 2002 Proton-transfer mechanism of pyridoxal enzymes studied by 3D-free energy profile

1999 - 2000 The proton-transfer mechanism of the catalytic reactions of pyridoxal enzymes

1998 - 1999 Development and application of a novel genetic strategy for visualization of functional neural pathways

1996 - 1996 タンパク質内部高秩序環境場におけるピリドキサールリン酸の光反応ダイナミックス

1994 - 1996 Substrate-Recognition Mechanism of Aromatic Amino Acid Aminotransferase

1995 - 1995 タンパク質内部高秩序環境場におけるピリドキサールリン酸の光反応ダイナミックス

1994 - 1995 高品質結晶によるアミノ基転移酵素の反応中間体の高分解能構造解析

1994 - 1994 タンパク質内部高秩序環境場におけるピリドキサールリン酸の光反応ダイナミックス

1993 - 1993 芳香族L-アミノ酸デカルボキシラーゼの構造と機能の解析

1990 - 1992 Structure and Function of Aspartate Aminotransferase

計算化学による酵素反応の理論的研究

酵素反応機構の速度論的解析

酵素・蛋白質機能の分子機構

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Papers (146)：

Mitsuo Shoji, Takeshi Murakawa, Shota Nakanishi, Mauro Boero, Yasuteru Shigeta, Hideyuki Hayashi, Toshihide Okajima. Molecular mechanism of a large conformational change of the quinone cofactor in the semiquinone intermediate of bacterial copper amine oxidase. CHEMICAL SCIENCE. 2022. 13. 36. 10923-10938
Takeshi Murakawa, Mamoru Suzuki, Toshi Arima, Michihiro Sugahara, Tomoyuki Tanaka, Rie Tanaka, So Iwata, Eriko Nango, Kensuke Tono, Hideyuki Hayashi, et al. Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase. Acta Crystallographica Section F Structural Biology Communications. 2021. 77. 10
Takeshi Murakawa, Kazuo Kurihara, Mitsuo Shoji, Chie Shibazaki, Tomoko Sunami, Taro Tamada, Naomine Yano, Taro Yamada, Katsuhiro Kusaka, Mamoru Suzuki, et al. Neutron crystallography of copper amine oxidase reveals keto/enolate interconversion of the quinone cofactor and unusual proton sharing. Proceedings of the National Academy of Sciences of the United States of America. 2020. 117. 20. 10818-10824
Yasuhiro Machida, Takeshi Murakawa, Akiko Sakai, Mitsuo Shoji, Yasuteru Shigeta, Hideyuki Hayashi. Reaction of threonine synthase with the substrate analogue 2-amino-5-phosphonopentanoate: implications into the proton transfer at the active site. Journal of biochemistry. 2020. 167. 4. 357-364
Mitsuo Shoji, Takeshi Murakawa, Mauro Boero, Yasuteru Shigeta, Hideyuki Hayashi, Toshihide Okajima. Unique protonation states of aspartate and topaquinone in the active site of copper amine oxidase. RSC Advances. 2020. 10. 63. 38631-38639

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MISC (61)：

村川武志, 馬場清喜, 河野能顕, 林秀行, 矢野貴人, 熊坂崇, 山本雅貴, 谷澤克行, 岡島俊英. 銅含有アミン酸化酵素触媒機構の“in crystallo“熱力学的解析. 日本細胞生物学会大会(Web). 2019. 71st
岡島俊英, 村川武志, 栗原和男, 安達基泰, 柴崎千枝, 玉田太郎, 林秀行, 谷澤克行. Active-site structure of copper amine oxidase determined by high-resolution X-ray and neutron crystallography. 量子ビームサイエンスフェスタ(Web). 2017. 2016
村川武志, 宮原郁子, 林秀行. 5.アスパラギン酸アミノトランスフェラーゼとマロン酸の複合体による超閉構造について(第442回研究協議会研究発表要旨,ビタミンB研究委員会). ビタミン. 2016. 90. 1. 43-44
主馬野祐希, 湯川直樹, 生城浩子, 林秀行, 矢野貴人, 神谷信夫, 宮原郁子. 1-III-11 セリンヒドロキシメチル基転移酵素のキノノイド中間体の構造(一般演題要旨,ビタミン・バイオファクター研究のさらなる魅力〜大和まほろぱからの発信〜,第67回大会講演要旨). ビタミン. 2015. 89. 4. 228-228
赤井翔太, 生城浩子, 澤井大樹, 林秀行, 矢野貴人, 神谷信夫, 宮原郁子. 1-IV-4 高度好熱菌由来ホモセリン脱水素酵素の三元複合体結晶構造解析(一般演題要旨,第66回大会講演要旨). ビタミン. 2014. 88. 4. 229-229

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Books (8)：

速度論的解析に基づく反応機構
タンパク質科学(化学同人) 2005
酵素触媒の原理
タンパク質の分子設計(共立出版) 2001
Branched-chain amino-acid aminotransferase of Escherichia coli
Methods. Enzymol. 2000
Branched-chain amino-acid aminotransferase of Escherichia coli
Methods. Enzymol. 2000
タンパク質-科学と工学
講談社 1999

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Education (2)：

- 1986 Osaka University Graduate School of Medicine Physiology
- 1982 Osaka University Faculty of Medicine Division of Medicine

Professional career (1)：

Doctor of Medical Science (Osaka University)

Work history (7)：

2024/04 - 現在 Senri Kinran University Faculty of Nursing Professor
2021/04 - 2023/03 Osaka Medical and Pharmaceutical University Faculty of Medicine Professor
2004/04 - 2021/03 Osaka Medical College Faculty of Medicine Professor
1994/04 - 2004/03 Osaka Medical College Faculty of Medicine Senior Associate Professor
1988/02 - 1994/02 Osaka Medical College Faculty of Medicine Associate Professor

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Awards (2)：

2022/06 - The Vitamin Society of Japan Award of the Vitamin Society of Japan Structure and function of vitamin B6 enzymes
1998 - The Vitamin Society of Japan Young Scientist Award of the Vitamin Society of Japan Refined reaction mechanism of aspartate aminotransferase

Association Membership(s) (9)：

THE CHEMICAL SOCIETY OF JAPAN , 日本ビタミン学会 , 日本蛋白質科学会 , アメリカ化学会(American Chemical Society) , 日本生化学会 , Protein Society of Japan , American Chemical Society , Japan Society of Vitaminology , Japanese Biochemical Society

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