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J-GLOBAL ID：200902228210002378   Reference number：09A1253329

Mutagenesis of longer inserts by the ligation of two PCR fragments amplified with a mutation primer

変異プライマーで増幅した2つのPCR断片のライゲーションによる長インサートの変異誘発

Author (5)： KATO Yu (Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City Univ., Suehiro-cho 1-7-29 ...) ,  ARAKAWA Noriaki (Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City Univ., Suehiro-cho 1-7-29 ...) ,  MASUISHI Yusuke (Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City Univ., Suehiro-cho 1-7-29 ...) ,  KAWASAKI Hiroshi (Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City Univ., Suehiro-cho 1-7-29 ...) ,  HIRANO Hisashi (Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City Univ., Suehiro-cho 1-7-29 ...)
Material： Journal of Bioscience and Bioengineering
Volume： 107  Issue： 1  Page： 95-97  Publication year： Jan. 2009 
JST Material Number： G0535B  ISSN： 1389-1723  CODEN： JBBIF6  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： *mutagenesis ,...

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JST classification (2)： Research methods in biological sciences in general  (EA03010K) ,  Molecular genetics in general  (EC02010J)

Reference (12)：

• LING, M. M. Approaches to DNA mutagenesis : an overview. Anal. Biochem. 1997, 254, 157-178
• MIYASHITA, M. Mutational analysis and localization of the inositol transporters of Saccharomyces cerevisiae. J. Biosci. Bioeng. 2003, 96, 291-297
• HOSOMI, N. Site-directed mutagenesis study of the antibody 2D7 which catalyzes a reaction for insertion of Cu^<2+> into mesoporphyrin. J. Biosci. Bioeng. 2005, 99, 222-229
• LI, M. Z. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nature. 2007, 4, 251-256
• IMAI, Y. simple and rapid method for generating a deletion by PCR. Nucleic Acids Res. 1991, 19, 2785

Terms in the title (8)： 変異 ,  プライマー ,  増幅 ,  PCR ,  断片 ,  ライゲーション ,  インサート ,  変異誘発