Art

J-GLOBAL ID：200902241925994129   Reference number：08A0581786

Refolding of a recombinant organic solvent-stable lipase, which is overexpressed and forms an inclusion body, and activation with lipase-specific foldase

過剰発現されて封入体を形成する組換え型有機溶媒安定性リパーゼのリフォールディングとリパーゼ特異的フォールダーゼによる活性化

Author (6)： OGINO Hiroyasu (Dep. of Chemical Engineering, Osaka Prefecture Univ., 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, JPN) ,  INOUE Sosuke (Dep. of Chemical Engineering, Osaka Prefecture Univ., 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, JPN) ,  AKAGI Rieko (Dep. of Chemical Engineering, Osaka Prefecture Univ., 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, JPN) ,  YASUDA Masahiro (Dep. of Chemical Engineering, Osaka Prefecture Univ., 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, JPN) ,  DOUKYU Noriyuki (Dep. of Life Sciences, Toyo Univ., Oura-gun, Gunma 374-0193, JPN) ,  ISHIMI Kosaku (Dep. of Chemical Engineering, Osaka Prefecture Univ., 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, JPN)
Material： Biochemical Engineering Journal  (Biochem Eng J)
Volume： 40  Issue： 3  Page： 507-511  Publication year： Jul. 01, 2008 
JST Material Number： W1014A  ISSN： 1369-703X  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： *lipase ,  gene expression ,  transformation ,  Escherichia coli ,  Pseudomonas aeruginosa ,  *inclusion body ,  organic solvent ,  activation ,  recombinant protein ,  signal peptide ,  solubilization ,  enzyme production ,  chaperone protein ,  promoter(genetics) ,  enzyme ,  *folding(morphology)
Semi thesaurus term： Escherichia coli ,  Pseudomonas aeruginosa ,  T7プロモーター ,  Chaperonins ,  フォールダーゼ ,  refolding

JST classification (1)： Microbial enzyme production  (FK03030L)

Reference (26)：

• H. Ogino, Organic solvent-stable enzymes, in: K.S. Siddiqui, T. Thomas (Eds.), Protein Adaptation in Extremophiles, Nova Science Publishers, Inc., New York, in press.
• H. Ogino, H. Ishikawa, Enzymes which are stable in the presence of organic solvents, J. Biosci. Bioeng. 91 (2001) 109-116.
• H. Ogino, K. Miyamoto, H. Ishikawa, Organic-solvent-tolerant bacterium which secretes organic-solvent-stable lipolytic enzyme, Appl. Environ. Microbiol. 60 (1994) 3884-3886.
• H. Ogino, K. Miyamoto, M. Yasuda, K. Ishimi, H. Ishikawa, Growth of organic solvent-tolerant Pseudomonas aeruginosa LST-03 in the presence of various organic solvents and production of lipolytic enzyme in the presence of cyclohexane, Biochem. Eng. J. 4 (1999) 1-6.
• H. Ogino, S. Nakagawa, K. Shinya, T. Muto, N. Fujimura, M. Yasuda, H. Ishikawa, Purification and characterization of organic solvent-stable lipase from organic solvent-tolerant Pseudomonas aeruginosa LST-03, J. Biosci. Bioeng. 89 (2000) 451-457.

Terms in the title (8)： 過剰発現 ,  封入体 ,  組換え型 ,  有機溶媒 ,  安定性 ,  リパーゼ ,  リフォールディング ,  活性化