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J-GLOBAL ID：200902250648716930   Reference number：04A0111769

Substrate Hydroxyl Groups Are Involved in the Ionization of Catalytic Carboxyl Groups of Aspergillus niger α-Glucosidase

基質水酸基がAspergillus niger α-グルコシダーゼの活性中心カルボキシル基の解離に与える影響

Author (9)： OGAWA M (Nihon Univ., Fujisawa, JPN) ,  NISHIO T (Nihon Univ., Fujisawa, JPN) ,  HAKAMATA W (National Inst. Health Sci., Tokyo, JPN) ,  MATSUISHI Y (Nihon Univ., Fujisawa, JPN) ,  HOSHINO S (Nihon Univ., Fujisawa, JPN) ,  KONDO A (Nihon Univ., Fujisawa, JPN) ,  KITAGAWA M (Nihon Univ., Fujisawa, JPN) ,  KAWACHI R (Nihon Univ., Fujisawa, JPN) ,  OKU T (Nihon Univ., Fujisawa, JPN)
Material： Journal of Applied Glycoscience  (応用糖質科学)
Volume： 51  Issue： 1  Page： 9-14  Publication year： Jan. 20, 2004 
JST Material Number： F0614A  ISSN： 1344-7882  CODEN： JAGLFX  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *glucosidase ,...
Semi thesaurus term： *Aspergillus niger ,...

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JST classification (1)： Enzyme in general  (EB09010D)

Substance index (5)： p-Nitrophenyl α-glucoside ,  1-O-Methyl-β-maltose ,  4-Nitrophenyl 2-deoxy-α-D-glucopyranoside ,  4-Nitrophenyl 3-deoxy-α-D-glucopyranoside ,  Methyl 6-O-(2-deoxy-α-D-glucopyranosyl)-β-D-glucopyranoside

Reference (36)：

• 1) T.P. Frandsen, B.B. Stoffer, M.M. Palcic, S. Hof and B. Svensson: Structure and energetics of the glucoamylase-isomaltose transition-state complex probed by using modeling and deoxygenated substrates coupled with site-directed mutagenesis. J. Mol. Biol., 263, 79-89 (1996).
• 2) R.U. Lemieux, U. Spohr, M. Bach, D.R. Cameron, T.P. Frandsen, B.B. Stoffer, B. Svensson and M.M. Palcic: Chemical mapping of the active site of the glucoamylase of Aspergillus niger. Can. J. Chem., 74, 319-335 (1996).
• 3) K. Bock and H. Pedersen: The substrate specificity of the enzyme amyloglucosidase (AMG). Part I. deoxy derivatives. Acta Chem. Scand., B41, 617-628 (1987).
• 4) M.R. Sierks, K. Bock, S. Refn and B. Svensson: Active site similarities of glucose dehydrogenase, glucose oxidase, and glucoamylase probed by deoxygenated substrates. Biochemistry, 31, 8972-8977 (1992).
• 5) W. Hakamata, T. Nishio and T. Oku: Synthesis of p-nitrophenyl 3- and 6-deoxy-α-D-glucopyranosides and their specificity to rice α-glucosidase. J. Appl. Glycosci., 46, 459-463 (1999).

Terms in the title (7)： 基質 ,  水酸基 ,  Aspergillus ,  α-グルコシダーゼ ,  活性中心 ,  カルボキシル基 ,  解離