Art

J-GLOBAL ID：200902265537064809   Reference number：08A0360026

Fluorescence Labeling of a Cytokine with Desthiobiotin-Tagged Fluorescent Puromycin

デスチオビオチンを付けた蛍光標識ピューロマイシンによるサイトカインの蛍光標識

Author (8)： SAKAMOTO Akihiko (Graduate School of Pharmaceutical Sciences, The Univ. of Tokyo) ,  YAMAGISHI Mai (Graduate School of Pharmaceutical Sciences, The Univ. of Tokyo) ,  WATANABE Takafumi (Graduate School of Advanced Sci. and Engineering, Waseda Univ.) ,  AIZAWA Youichi (Graduate School of Advanced Sci. and Engineering, Waseda Univ.) ,  KATO Takashi (Graduate School of Advanced Sci. and Engineering, Waseda Univ.) ,  KATO Takashi (School of Education, Waseda Univ.) ,  FUNATSU Takashi (Graduate School of Pharmaceutical Sciences, The Univ. of Tokyo) ,  FUNATSU Takashi (Center for NanoBio Integration, The Univ. of Tokyo)
Material： Journal of Bioscience and Bioengineering  (J Biosci Bioeng)
Volume： 105  Issue： 3  Page： 238-242 (J-STAGE)  Publication year： 2008 
JST Material Number： G0535B  ISSN： 1389-1723  CODEN： JBBIF6  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *fluorescent labeling ,...
Semi thesaurus term： tandem ,...

Register(free) or Log In to access     To see more with JDream III (charged).   {{ this.onShowAbsJLink("http://jdream3.com/lp/jglobal/index.html?docNo=08A0360026&from=J-GLOBAL&jstjournalNo=G0535B") }}

JST classification (1)： Research methods in biological sciences in general  (EA03010K)

Substance index (3)： Dethiobiotin ,  Histidine ,  Puromycin

Reference (10)：

• KATO, T. Native thrombopoietin : structure and function. Stem Cells. 1998, 16, 322-328
• NEMOTO, N. Fluorescence labeling of the C-terminus of proteins with a puromycin analogue in cell-free translation systems. FEBS Lett. 1999, 462, 43-46
• YAMAGUCHI, J. Rapid functional analysis of protein-protein interactions by fluorescent C-terminal labeling and single-molecule imaging. FEBS Lett. 2001, 502, 79-83
• OYAMA, R. Protein-protein interaction analysis by C-terminally specific fluorescence labeling and fluorescence cross-correlation spectroscopy. Nucleic Acids Res. 2006, 34, e102
• HIRSCH, J. D. Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins : uses for protein labeling, detection, and isolation. Anal. Biochem. 2002, 308, 343-357

Terms in the title (4)： デスチオビオチン ,  蛍光標識 ,  ピューロマイシン ,  サイトカイン