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J-GLOBAL ID：200902282734600315   Reference number：05A0716148

Safe Specimen Preparation for Electron Microscopy of Pathogenic Fungi by Freeze-substitution after Glutaraldehyde Fixation

グルタルアルデヒド固定後の凍結-置換による病原性真菌の電子顕微鏡観察のための安全な検体調製

Author (4)： YAMAGUCHI Masashi (Chiba Univ., Chiba, JPN) ,  OHKUSU Misako (Chiba Univ., Chiba, JPN) ,  SAMESHIMA Masazumi (Hirosaki Univ., Aomori, JPN) ,  KAWAMOTO Susumu (Chiba Univ., Chiba, JPN)
Material： 日本医真菌学会雑誌  (Japanese Journal of Medical Mycology)
Volume： 46  Issue： 3  Page： 187-192  Publication year： Jul. 30, 2005 
JST Material Number： G0589A  ISSN： 0916-4804  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *electron microscopy ,...
Semi thesaurus term： *Eumycetes ,...

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JST classification (3)： Microscopic methods of living organisms  (EA03050C) ,  Microorganism test methods  (EG02020G) ,  Microorganism tests  (GC02030H)

Substance index (1)： Glutaraldehyde

Reference (16)：

• OSUMI, M. The ultrastructure of yeast : cell wall structure and formation. Micron. 1998, 29, 207-233
• YAMAGUCHI, M. Electron microscopy of hepatitis B virus core antigen expressing yeast cells by freeze-substitution fixation. Eur J Cell Biol. 1988, 47, 138-143
• YAMAGUCHI, M. Dynamics of hepatitis B virus core antigen in a transformed yeast cell : analysis with an inducible system. J Electron Microsc. 1994, 43, 386-393
• YAMAGUCHI, M. Translocation of hepatitis B virus core particles through nuclear pores in transformed yeast cells. J Electron Microsc. 1996, 45, 321-324
• BISWAS, SK. Quantitative three-dimensional structural analysis of Exophiala dermatitidis yeast cells by freeze-substitution and serial ultrathin sectioning. J Electron Microsc. 2003, 52, 133-143

Terms in the title (7)： グルタルアルデヒド ,  凍結 ,  置換 ,  病原性真菌 ,  電子顕微鏡観察 ,  検体 ,  調製