Art

J-GLOBAL ID：201002264158797178   Reference number：10A0188611

Substitution of Glu122 by Glutamine Revealed the Function of the Second Water Molecule as a Proton Donor in the Binuclear Metal Enzyme Creatininase

二核金属酵素クレアチニナーゼにおけるプロトンドナーとして第二水分子の機構を明らかにしたGlu122のグルタミンによる置換

Author (10)： YAMASHITA Kinuyo (Graduate School of Biomedical Sciences, Nagasaki Univ., 1-14 Bunkyo-machi, Nagasaki 852-8521, JPN) ,  NAKAJIMA Yoshitaka (Graduate School of Biomedical Sciences, Nagasaki Univ., 1-14 Bunkyo-machi, Nagasaki 852-8521, JPN) ,  MATSUSHITA Hayato (Graduate School of Biomedical Sciences, Nagasaki Univ., 1-14 Bunkyo-machi, Nagasaki 852-8521, JPN) ,  NISHIYA Yoshiaki (Tsuruga Inst. of Biotechnology, Toyobo, Co. Ltd., 10-24 Toyo-cho, Tsuruga, Fukui 914-0047, JPN) ,  YAMAZAWA Ryuji (Graduate School of Biomedical Sciences, Nagasaki Univ., 1-14 Bunkyo-machi, Nagasaki 852-8521, JPN) ,  WU Yu-fan (Graduate School of Biomedical Sciences, Nagasaki Univ., 1-14 Bunkyo-machi, Nagasaki 852-8521, JPN) ,  MATSUBARA Futoshi (Graduate School of Biomedical Sciences, Nagasaki Univ., 1-14 Bunkyo-machi, Nagasaki 852-8521, JPN) ,  OYAMA Hiroshi (Graduate School of Biomedical Sciences, Nagasaki Univ., 1-14 Bunkyo-machi, Nagasaki 852-8521, JPN) ,  ITO Kiyoshi (Graduate School of Biomedical Sciences, Nagasaki Univ., 1-14 Bunkyo-machi, Nagasaki 852-8521, JPN) ,  YOSHIMOTO Tadashi (Graduate School of Biomedical Sciences, Nagasaki Univ., 1-14 Bunkyo-machi, Nagasaki 852-8521, JPN)
Material： Journal of Molecular Biology  (J Mol Biol)
Volume： 396  Issue： 4  Page： 1081-1096  Publication year： Mar. 05, 2010 
JST Material Number： D0124B  ISSN： 0022-2836  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： *amide hydrolase ,  crystal structure ,  structure analysis ,  amino acid residue ,  *water molecule ,  enzyme inhibitor ,  subunit ,  protein complex ,  site-directed mutagenesis ,  substrate (biochemistry) ,  lactam ,  hydrophobic ,  conformational transition ,  enzyme activity ,  hydroxide ion ,  mutant ,  zinc chloride ,  manganese chloride ,  atomic absorption spectrometry ,  proton shift ,  *dinuclear complex ,  metalloenzyme ,  donor ,  protonation ,  carboxy group ,  *creatininase
Semi thesaurus term： carboxyl group

JST classification (2)： Molecular structure  (EB03020Y) ,  Enzyme in general  (EB09010D)

Substance index (2)： Methylguanidine ,  Creatinine

Terms in the title (8)： 核 ,  金属酵素 ,  クレアチニナーゼ ,  プロトン ,  ドナー ,  水分子 ,  グルタミン ,  置換