Art

J-GLOBAL ID：201002289337642953   Reference number：10A0609460

Development of Triplex SYBR Green Real-Time PCR for Detecting Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp. without Extraction of DNA

Mycoplasma pneumoniae,Chlamydophila pneumoniae,Legionella属を検出するためのDNA抽出不要の3重SYBRグリーンリアルタイムPCR法の開発

Author (11)： KERDSIN Anusak (National Inst. Health, Ministry of Public Health, Nonthaburi) ,  UCHIDA Ryuichi (Osaka Univ., Osaka) ,  VERATHAMJAMRUS Chris (National Inst. Health, Ministry of Public Health, Nonthaburi) ,  PUANGPATRA Parichart (National Inst. Health, Ministry of Public Health, Nonthaburi) ,  KAWAKAMI Kazuyoshi (Tohoku Univ. Graduate School of Medicine, Miyagi) ,  PUNTANAKUL Pollert (Bangkok Metropolitan Administration Medical Coll., Bangkok) ,  LOCHINDARAT Sorasak (Queen Sirikit National Inst. Child Health, Bangkok, THA) ,  BUNNAG Thanyanut (Queen Sirikit National Inst. Child Health, Bangkok, THA) ,  SAWANPANYALERT Pathom (National Inst. Health, Ministry of Public Health, Nonthaburi) ,  DEJSIRILERT Surang (National Inst. Health, Ministry of Public Health, Nonthaburi) ,  OISHI Kazunori (Osaka Univ., Osaka)
Material： Japanese Journal of Infectious Diseases  (日本感染症雑誌)
Volume： 63  Issue： 3  Page： 173-180  Publication year： May. 31, 2010 
JST Material Number： F0552A  ISSN： 1344-6304  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： Mycoplasma pneumoniae ,...
Semi thesaurus term： Chlamydophila pneumoniae ,...

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JST classification (3)： Diagnostics of infectious diseases,parasitic diseases.  (GD01020S) ,  Respiratory system diagnosis  (GI02000S) ,  Research methods in genetics  (EC01020N)

Reference (21)：

• File, T. M.(2003): Community-acquired pneumonia. Lancet, 362, 1991-2001.
• Khanna, M., Fan, J., Pehler-Harrington, K., et al.(2005): The pneumoplex assays, a multiplex PCR-enzyme hybridization assay that allows simultaneous detection of five organisms, Mycoplasma pneumoniae, Chlamydia (Chlamydophila) pneumoniae, Legionella pneumophila, Legionella micdadei, and Bordetella pertussis, and its real-time counterpart. J. Clin. Microbiol., 43, 565-571.
• Muder, R. R. and Yu, V. L.(2002): Infection due to Legionella species other than L. pneumophila. Clin. Infect. Dis., 35, 990-998.
• Morozumi, M., Nakayama, E., Iwata, S., et al.(2006): The Acute Respiratory Diseases Study Group. Simultaneous detection of pathogens in clinical samples from patients with community acquired pneumonia by real-time PCR with pathogen-specific molecular beacon probes. J. Clin. Microbiol., 44, 1440-1446.
• Welti, M., Jaton, K., Altwegg, M., et al.(2003): Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions. Diagn. Microbiol. Infect. Dis., 45, 85-95.

Terms in the title (8)： Mycoplasma ,  Chlamydophila ,  Legionella属 ,  DNA抽出 ,  3重 ,  グリーン ,  リアルタイムPCR法 ,  開発