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J-GLOBAL ID：201402247622606434   Reference number：14A1012922

One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System

CRISPR/Cas9システムによる遺伝的変異アレルを持つ表現型発現トリプルノックアウトマウスのワンステップ作出

Author (4)： FUJII Wataru (Dep. of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo, Tokyo ...) ,  ONUMA Asuka (Dep. of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo, Tokyo ...) ,  SUGIURA Koji (Dep. of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo, Tokyo ...) ,  NAITO Kunihiko (Dep. of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The Univ. of Tokyo, Tokyo ...)
Material： Journal of Reproduction and Development  (J Reprod Dev)
Volume： 60  Issue： 4  Page： 324-327 (J-STAGE)  Publication year： 2014 
JST Material Number： F0766A  ISSN： 0916-8818  Document type： Article
Article type： 解説  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *palindrome ,...
Semi thesaurus term： *variation(biology) ,...

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JST classification (5)： Laboratry organisms  (EA03070Y) ,  Structure and chemistry of genes  (EC02020U) ,  Gene expression  (EC02040Q) ,  Genetic variation  (EC01030Y) ,  Gene manipulation  (EC02050B)

Reference (12)：

• 1. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Multiplex genome engineering using CRISPR/Cas systems. Science 2013; 339: 819-823.
• 2. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. RNA-guided human genome engineering via Cas9. Science 2013; 339: 823-826.
• 3. Fujii W, Kawasaki K, Sugiura K, Naito K. Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Nucleic Acids Res 2013; 41: e187.
• 4. Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell 2013; 153: 910-918.
• 5. Li W, Teng F, Li T, Zhou Q. Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nat Biotechnol 2013; 31: 684-686.

Terms in the title (6)： CRISPR ,  Cas9 ,  遺伝的変異 ,  アレル ,  表現型発現 ,  トリプルノックアウトマウス