Art

J-GLOBAL ID：201402259641677235   Reference number：14A0324533

Identification of Site-Specific Degradation in Bacterially Expressed Human Fibroblast Growth Factor 4 and Generation of an Aminoterminally Truncated, Stable Form

細菌で発現されたヒト線維芽細胞増殖因子4における部位特異的分解の同定並びにアミノ末端で短縮された安定型の生成

Author (7)： SUGAWARA Saiko (Graduate School of Bioresource Sciences, Akita Prefectural Univ., 010-0195, Akita, JPN) ,  ITO Toshihiko (Graduate School of Bioresource Sciences, Akita Prefectural Univ., 010-0195, Akita, JPN) ,  SATO Shiori (Graduate School of Bioresource Sciences, Akita Prefectural Univ., 010-0195, Akita, JPN) ,  SATO Yuki (Graduate School of Bioresource Sciences, Akita Prefectural Univ., 010-0195, Akita, JPN) ,  KASUGA Kano (Graduate School of Bioresource Sciences, Akita Prefectural Univ., 010-0195, Akita, JPN) ,  KOJIMA Ikuo (Graduate School of Bioresource Sciences, Akita Prefectural Univ., 010-0195, Akita, JPN) ,  KOBAYASHI Masayuki (Graduate School of Bioresource Sciences, Akita Prefectural Univ., 010-0195, Akita, JPN)
Material： Applied Biochemistry and Biotechnology. Part A. Enzyme Engineering and Biotechnology  (Applied Biochemistry and Biotechnology)
Volume： 172  Issue： 1  Page： 206-215  Publication year： Jan. 2014 
JST Material Number： E0344C  ISSN： 0273-2289  Document type： Article
Article type： 原著論文  Country of issue： United States (USA)  Language： ENGLISH (EN)

Thesaurus term： *FGF ,  proteolysis ,  stability ,  *recombinant protein ,  Escherichia coli ,  Amino Acid Sequence ,  human ,  pharmacological action ,  amino acid composition ,  SDS-PAGE ,  Western blotting ,  *FGF4 ,  Protein Stability ,  recombinant E. coli ,  cell growth promoting activity ,  N-terminal sequencing ,  microorganism ,  bioassay
Semi thesaurus term： 切断部位

JST classification (2)： Substance production by microorganisms,cultured tissues/cells in general  (FK03010P) ,  Gene manipulation  (EC02050B)

Terms in the title (7)： 細菌 ,  発現 ,  ヒト ,  線維芽細胞増殖因子4 ,  分解 ,  同定 ,  アミノ末端