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J-GLOBAL ID：201702285417127038   Reference number：17A0864456

ROCK INHIBITOR Y-27632 USED DURING CELL AGGREGATE FORMATION ADVERSELY AFFECTS THE EARLY CARDIAC DIFFERENTIATION OF HUMAN iPS CELLS

細胞凝集体形成時に使用するROCK阻害剤Y-27632はヒトiPS細胞の早期心分化に対して悪影響を及ぼす

Author (3)： OHNUKI Yoshitsugu (Faculty of Life and Environmental Sciences, Graduate Faculty of Interdisciplinary Research, University of Yamanashi) ,  KONDO Takumi (Faculty of Life and Environmental Sciences, Graduate Faculty of Interdisciplinary Research, University of Yamanashi) ,  KUROSAWA Hiroshi (Faculty of Life and Environmental Sciences, Graduate Faculty of Interdisciplinary Research, University of Yamanashi)
Material： 組織培養研究  (Journal of Experimental & Applied Cell Culture Research)
Volume： 36  Issue： 2  Page： 21-29(J-STAGE)  Publication year： 2017 
JST Material Number： Z0362B  ISSN： 0912-3636  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *enzyme inhibitor ,...
Semi thesaurus term： cell physiology ,...

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Author keywords (4)： ROCK inhibitor ,  Y-27632 ,  iPS cells ,  cardiac differentiation

JST classification (3)： Cell and tissue culture methods  (EA03040R) ,  Heart  (EJ04020D) ,  Applications of enzymes  (EB09100B)

Substance index (1)： Y-27632

Reference (21)：

• 1) Riento, K. and Ridley, A. J.: ROCKs: Multifunctional kinases in cell behaviour. Rev. Mol. Cell Biol., 4, 446-456, 2003.
• 2) Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M. and Narumiya, S.: Pharmacological properties of Y-27632, a specific inhibitor of Rho-associated kinase. Mol. Pharm., 57, 976-983, 2000.
• 3) Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J. B., Nishikawa, S., Nishikawa, S., Muguruma, K. and Sasai, Y.: A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat. Biotechnol., 25, 681-686, 2007.
• 4) Osakada, F., Jin, Z., Hirami, Y., Ikeda, H., Danjyo, T., Watanabe, K., Sasai, Y. and Takahashi, M.: In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction. J. Cell Sci., 122, 3169-3179, 2009.
• 5) Horiguchi, A., Yazaki, K., Aoyagi, M., Ohnuki, Y. and Kurosawa, H.: Effective Rho-associated protein kinase inhibitor treatment to dissociate human iPS cells for suspension culture to form embryoid body-like cell aggregates. J. Biosci. Bioeng., 118, 588-592, 2014.

Terms in the title (6)： 細胞凝集体 ,  ROCK阻害剤 ,  Y-27632 ,  ヒトiPS細胞 ,  早期 ,  分化