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J-GLOBAL ID：201802211314262798   Reference number：18A1106082

Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes

ブタ卵母細胞におけるゲノム編集効率に影響する重要因子としての活性化後のCRISPR/Cas9関連mRNAマイクロインジェクションのタイミング【JST・京大機械翻訳】

Author (11)： Sato Masahiro (Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima 890-8544, Japan) ,  Kosuke Maeda (Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan) ,  Koriyama Miyu (Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan) ,  Inada Emi (Department of Pediatric Dentistry, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan) ,  Saitoh Issei (Division of Pediatric Dentistry, Department of Oral Health Sciences, Course for Oral Life Science, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8514, Japan) ,  Ohtsuka Masato (Division of Basic Molecular Science and Molecular Medicine, School of Medicine, Tokai University, Kanagawa 259-1193, Japan) ,  Ohtsuka Masato (The Institute of Medical Sciences, Tokai University, Kanagawa 259-1193, Japan) ,  Nakamura Shingo (Division of Biomedical Engineering, National Defense Medical College Research Institute, Saitama 359-8513, Japan) ,  Sakurai Takayuki (Basic Research Division for Next-Generation Disease Models and Fundamental Technology, Research Center for Next Generation Medicine, Shinshu University, Nagano 390-8621, Japan) ,  Watanabe Satoshi (Animal Genome Research Unit, Division of Animal Science, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan) ,  Miyoshi Kazuchika (Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan)
Material： Theriogenology
Volume： 108  Page： 29-38  Publication year： 2018 
JST Material Number： A1189A  ISSN： 0093-691X  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Abstract/Point： Recently, successful one-step ...

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Thesaurus term： mosaic ,  fluorescent labeling ,  epitope ,  endonuclease ,  *mRNA ,  gene ,  galactosyltransferase ,  Cytoplasm ,  blastocyst ,  *Oocytes ,  *microinjection ,  Mutation ,  Blastomeres ,  *Cas9 protein ,  *Gene Editing
Semi thesaurus term： *Cas9 ,  *ゲノムエディティング , 【Automatic Indexing@JST】

Author keywords (9)： α-Gal epitope ,  α-1 3-Galactosyltransferase ,  CRISPR/Cas9 ,  Microinjection ,  mRNA ,  Mosaicism ,  Biallelic KO ,  Indels ,  Isolectin BS-I-B₄

JST classification (1)： Gene manipulation  (EC02050B)

Terms in the title (9)： ブタ ,  卵母細胞 ,  ゲノム編集 ,  因子 ,  活性化 ,  CRISPR ,  Cas9 ,  mRNA ,  マイクロインジェクション