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J-GLOBAL ID：201802226794044477   Reference number：18A1867632

Subtle changes in host cell density cause a serious error in monitoring of the intracellular growth of Chlamydia trachomatis in a low-oxygen environment: Proposal for a standardized culture method

宿主細胞密度の微妙な変化は低酸素環境におけるChlamydia trachomatisの細胞内増殖のモニタリングにおいて重大な誤差を引き起こす:標準化培養法の提案【JST・京大機械翻訳】

Author (6)： Sakai Kouhei (Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan) ,  Matsuo Junji (Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan) ,  Watanabe Takanori (Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan) ,  Okubo Torahiko (Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan) ,  Nakamura Shinji (Division of Biomedical Imaging Research, Juntendo University Graduate School of Medicine, Tokyo 113-8421, Japan) ,  Yamaguchi Hiroyuki (Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan)
Material： Journal of Microbiological Methods  (J Microbiol Methods)
Volume： 153  Page： 84-91  Publication year： 2018 
JST Material Number： H0882A  ISSN： 0167-7012  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Abstract/Point： We monitored Chlamydia trachom...

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Thesaurus term： fibrous disease ,  *oxygen deficiency ,  culture condition ,  HeLa cell ,  Down-Regulation ,  cell culture ,  gene ,  *Chlamydia trachomatis ,  gene expression ,  bacterial infection (phenomenon) ,  bacterial growth ,  Dulbecco's Modified Eagle's Medium ,  *host cell ,  *microorganism ,  molecular genetic phenomenon ,  aliphatic amine ,  thiol ,  aliphatic carboxylic acid ,  tripeptide ,  sulfur containing amino acid ,  hexose ,  aldose

Author keywords (6)： Chlamydia trachomatis ,  Low-oxygen environment ,  Host cell density ,  RPMI ,  DMEM ,  CTGF

JST classification (2)： Microorganism test methods  (EG02020G) ,  Physiology and pathogenicity of microbial infection  (EG03053T)

Substance index (2)： Glutathione ,  Glucose

Terms in the title (10)： 宿主 ,  細胞密度 ,  低酸素環境 ,  Chlamydia ,  細胞内増殖 ,  モニタリング ,  誤差 ,  標準化 ,  培養法 ,  提案