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J-GLOBAL ID：201802278767343792   Reference number：18A0087675

A novel, simplified strategy of relative quantification N-glycan: Quantitative glycomics using electrospray ionization mass spectrometry through the stable isotopic labeling by transglycosylation reaction of mutant enzyme Endo-M-N175Q

相対的定量化N-グリカンの新規,単純化した戦略:変異酵素エンドM N175Qのグリコシル基転移反応による安定同位体標識によるエレクトロスプレイイオン化質量分析を用いた定量的グリコミクス【Powered by NICT】

Author (10)： Shi Qing (Key Laboratory for Natural Resource of Changbai Mountain & Functional Molecules, Ministry of Education, College of Pharmacy Yanbian University and Department of Pharmacy, Yanbian University Hospital, Yanji 133002, Jilin Province, China) ,  Hashimoto Ryugo (Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan) ,  Otsubo Tadamune (Department of Organic Chemistry, School of Pharmaceutical Sciences, Hiroshima International, University, Hiroshima, Japan) ,  Ikeda Kiyoshi (Department of Organic Chemistry, School of Pharmaceutical Sciences, Hiroshima International, University, Hiroshima, Japan) ,  Todoroki Kenichiro (Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan) ,  Mizuno Hajime (Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan) ,  Jin Dongri (Key Laboratory for Natural Resource of Changbai Mountain & Functional Molecules, Ministry of Education, College of Pharmacy Yanbian University and Department of Pharmacy, Yanbian University Hospital, Yanji 133002, Jilin Province, China) ,  Toyo'oka Toshimasa (Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan) ,  Jiang Zhe (Key Laboratory for Natural Resource of Changbai Mountain & Functional Molecules, Ministry of Education, College of Pharmacy Yanbian University and Department of Pharmacy, Yanbian University Hospital, Yanji 133002, Jilin Province, China) ,  Min Jun Zhe (Key Laboratory for Natural Resource of Changbai Mountain & Functional Molecules, Ministry of Education, College of Pharmacy Yanbian University and Department of Pharmacy, Yanbian University Hospital, Yanji 133002, Jilin Province, China)
Material： Journal of Pharmaceutical and Biomedical Analysis  (J Pharm Biomed Anal)
Volume： 149  Page： 365-373  Publication year： 2018 
JST Material Number： H0876A  ISSN： 0731-7085  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Abstract/Point： The lack of a highly sensitive...

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Thesaurus term： high sensitivity ,  labeling method ,  linearity ,  *Electrospray Ionization Mass Spectrometry ,  quantitative analysis ,  Glycosylation ,  recovery rate ,  enzyme reaction ,  human ,  deuteration ,  reproducibility ,  *glycan ,  *Glycomics ,  *N-glycan ,  liquid chromatography-tandem mass spectrometry
Semi thesaurus term： LC/MS/MS , 【Automatic Indexing@JST】

Author keywords (6)： N-glycan ,  Relative quantification ,  Endo-M-N175Q ,  Stable isotope labeling ,  LC-ESI-MS ,  Glycomics

JST classification (1)： Analysis of drugs  (GV01040Q)

Terms in the title (8)： 定量化 ,  N-グリカン ,  戦略 ,  変異酵素 ,  安定同位体標識 ,  エレクトロスプレイイオン化質量分析 ,  定量 ,  グリコミクス