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J-GLOBAL ID：201802281841970156   Reference number：18A0975547

Immunocytochemistry and fluorescence imaging efficiently identify individual neurons with CRISPR/Cas9-mediated gene disruption in primary cortical cultures

免疫細胞化学と蛍光イメージングは初代皮質培養におけるCRISPR/Cas9仲介遺伝子破壊を伴う個々のニューロンを効率的に同定する【JST・京大機械翻訳】

Author (4)： Tsunematsu Hiroto (Cellular and Molecular Neurobiology, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan) ,  Uyeda Akiko (Cellular and Molecular Neurobiology, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan) ,  Yamamoto Nobuhiko (Cellular and Molecular Neurobiology, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan) ,  Sugo Noriyuki (Cellular and Molecular Neurobiology, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan)
Material： BMC Neuroscience (Web)
Volume： 18  Issue： 1  Page： 55  Publication year： 2017 
JST Material Number： U7309A  ISSN： 1471-2202  Document type： Article
Article type： 原著論文  Country of issue： United Kingdom (GBR)  Language： ENGLISH (EN)

Abstract/Point： Background CRISPR/Cas9 system ...

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Thesaurus term： antibody ,  *cortex(animal tissue) ,  plasmid ,  *gene disruption ,  transfection ,  *culture(biology) ,  mitosis ,  *fluorescence ,  genome ,  mutation ,  CRE binding protein
Semi thesaurus term： 蛋白質発現 ,  *Cas9 ,  *CRISPR配列 ,  *蛍光イメージング , 【Automatic Indexing@JST】

Author keywords (6)： CREB ,  FOS ,  CRISPR/Cas9 ,  Cortical neuron ,  Gene disruption ,  Immunocytochemistry

JST classification (1)： Gene manipulation  (EC02050B)

Reference (28)：

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Terms in the title (10)： 免疫細胞化学 ,  蛍光イメージング ,  皮質 ,  培養 ,  CRISPR ,  Cas9 ,  仲介 ,  遺伝子破壊 ,  ニューロン ,  同定