Research theme for competitive and other funds (1)：
2018 - 2020 Studies of blanched-chain amino acid aminotransferase and BCAA metabolism on S. cerevisiae
Papers (15)：
Shota Isogai, Akira Nishimura, Naoyuki Murakami, Natsuki Hotta, Atsushi Kotaka, Yoichi Toyokawa, Hiroki Ishida, Hiroshi Takagi. Improvement of valine and isobutanol production in sake yeast by Ala31Thr substitution in the regulatory subunit of acetohydroxy acid synthase. FEMS yeast research. 2023. 23
Masatoshi Tsukahara, Shota Isogai, Haruna Azuma, Keiko Tsukahara, Yoichi Toyokawa, Hiroshi Takagi. Characterization of a new Saccharomyces cerevisiae isolated from banana stems and its mutant with l-leucine accumulation for awamori brewing. Bioscience, biotechnology, and biochemistry. 2022
Shota Isogai, Tomonori Matsushita, Hiroyuki Imanishi, Jirasin Koonthongkaew, Yoichi Toyokawa, Akira Nishimura, Xiao Yi, Romas Kazlauskas, Hiroshi Takagi. High-Level Production of Lysine in the Yeast Saccharomyces cerevisiae by Rational Design of Homocitrate Synthase. Applied and environmental microbiology. 2021. 87. 15. e0060021
