Art

J-GLOBAL ID：201902248737286922   Reference number：19A1475241

Methodological improvement of fluorescein isothiocyanate peanut agglutinin (FITC-PNA) acrosomal integrity staining for frozen-thawed Japanese Black bull spermatozoa

凍結融解した黒毛和種雄牛精子に対するフルオレセインインイソチオシアナートナンキンマメ凝集素(FITC-PNA)先体完全性染色の方法論的改善

Author (8)： RAJABI-TOUSTANI Reza (Laboratory of Veterinary Theriogenology, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan) ,  AKTER Quzi Sharmin (Laboratory of Veterinary Theriogenology, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan) ,  ALMADALY Essam A. (Department of Theriogenology, Faculty of Veterinary Medicine, Kafrelsheikh University, El-Geish street, 33516 Kafrelsheikh, Egypt) ,  HOSHINO Yoichiro (Hida Beef Cattle Research Department, Gifu Prefectural Livestock Research Institute, Takayama 506-0101, Japan) ,  HOSHINO Yoichiro (Present address: Graduate School of Agriculture, Kyoto University, Kyoto 622-0203, Japan) ,  ADACHI Hiromichi (Hida Beef Cattle Research Department, Gifu Prefectural Livestock Research Institute, Takayama 506-0101, Japan) ,  MUKOUJIMA Koushi (Hida Beef Cattle Research Department, Gifu Prefectural Livestock Research Institute, Takayama 506-0101, Japan) ,  MURASE Tetsuma (Laboratory of Veterinary Theriogenology, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan)
Material： Journal of Veterinary Medical Science (Web)
Volume： 81  Issue： 5  Page： 694-702(J-STAGE)  Publication year： 2019 
JST Material Number： U1094A  ISSN： 1347-7439  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： Japanese Black Cattle ,  *Tissue Preservation ,  *Cryopreservation ,  spermatozoon ,  fluorescent labeling ,  *lectin ,  biological sample staining ,  artificial fertilization ,  *sperm cryopreservation ,  Acrosome ,  *Peanut Agglutinin ,  beef cattle ,  lactone ,  spiro-compound ,  phenolic compound ,  inorganic acid ester ,  organosulphur compound ,  polynuclear aromatic compound ,  organocyano compound
Semi thesaurus term： *Peanut Agglutinin

Author keywords (3)： acrosomal integrity ,  bull spermatozoa ,  FITC-PNA staining

JST classification (1)： Reproduction management  (FD01030C)

Substance index (1)： Fluorescein isothiocyanate

Reference (22)：

• 1. Almadaly, E., El-Kon, I., Heleil, B., Fattouh, S., Mukoujima, K., Ueda, T., Hoshino, Y., Takasu, M. and Murase, T. 2012. Methodological factors affecting the results of staining frozen-thawed fertile and subfertile Japanese Black bull spermatozoa for acrosomal status. Anim. Reprod. Sci. 136: 23-32.
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• 4. Gürler, H., Calisici, O. and Bollwein, H. 2015. Inter- and intra-individual variability of total antioxidant capacity of bovine seminal plasma and relationships with sperm quality before and after cryopreservation. Anim. Reprod. Sci. 155: 99-105.
• 5. Harayama, H., Nishijima, K., Murase, T., Sakase, M. and Fukushima, M. 2010. Relationship of protein tyrosine phosphorylation state with tolerance to frozen storage and the potential to undergo cyclic AMP-dependent hyperactivation in the spermatozoa of Japanese Black bulls. Mol. Reprod. Dev. 77: 910-921.

Terms in the title (11)： 凍結融解 ,  黒毛和種 ,  雄牛 ,  精子 ,  凝集素 ,  FITC ,  PNA ,  先体 ,  完全性 ,  染色 ,  方法論