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J-GLOBAL ID：201902283537612730   Reference number：19A2830025

Nested PCR Amplification Secures DNA Template Quality and Quantity in Real-time mCOP-PCR Screening for SMA

ネステッドPCR増幅はSMAのリアルタイムMCOP-PCRスクリーニングにおけるDNA鋳型品質と量を保証する

Author (14)： WIJAYA Yogik Onky Silvana (Kobe Univ. Graduate School of Medicine, Kobe, JPN) ,  NIBA Emma Tabe Eko (Kobe Univ. Graduate School of Medicine, Kobe, JPN) ,  AR ROCHMAH Mawaddah (Kobe Univ. Graduate School of Medicine, Kobe, JPN) ,  HARAHAP Nur Imma Fatimah (Kobe Univ. Graduate School of Medicine, Kobe, JPN) ,  AWANO Hiroyuki (Kobe Univ. Graduate School of Medicine, Kobe, JPN) ,  TAKESHIMA Yasuhiro (Hyogo Coll. of Medicine, Nishinomiya, JPN) ,  SAITO Toshio (National Hospital Organization Toneyama National Hospital, Toneyama, JPN) ,  SAITO Kayoko (Tokyo Women’s Medical Univ., Tokyo, JPN) ,  TAKEUCHI Atsuko (Kobe Pharmaceutical Univ., Kobe, JPN) ,  LAI Poh San (National Univ. Singapore, Singapore) ,  BOUIKE Yoshihiro (Kobe Gakuin Univ., Kobe, JPN) ,  NISHIO Hisahide (Kobe Univ. Graduate School of Medicine, Kobe, JPN) ,  NISHIO Hisahide (Kobe Gakuin Univ., Kobe, JPN) ,  SHINOHARA Masakazu (Kobe Univ. Graduate School of Medicine, Kobe, JPN)
Material： Kobe Journal of Medical Sciences (Web)  (Kobe J Med Sci (Web))
Volume： 65  Issue： 2  Page： E54-E58  Publication year： 2019 
JST Material Number： U0095A  ISSN： 1883-0498  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *polymerase chain reaction ,...
Semi thesaurus term： *nested PCR ,...

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JST classification (1)： Neurologic diagnosis  (GN02000H)

Reference (16)：

• Al-Soud, W.A., Jönsson, L.J., Râdström, P. 2000. Identification and characterization of Immunoglobulin G in blood as a major inhibitor of diagnostic PCR. J Clin Microbiol 38: 345-350.
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• Ar Rochmah, M., Harahap, N.I.F., Niba, E.T.E., et al. 2017. Genetic screening of spinal muscular atrophy using a real time modified COP-PCR technique with dried blood spot DNA. Brain Dev 39: 774-782.
• Burghes, A.H.M., Beattie, C.E. 2009. Spinal muscular atrophy: why do low levels of survival motor neuron protein make motor neurons sick? Nat Rev Neurosci 10:597-609.
• Finkel, R.S., Chiriboga, C.A., Vajsar, J., et al. 2016. Treatment of infantile-onset spinal muscular atrophy with nusinersen: a phase 2, open-label, dose-escalation study. Lancet 388:3017-3026

Terms in the title (9)： ネステッドPCR ,  増幅 ,  SMA ,  リアルタイム ,  PCR ,  スクリーニング ,  DNA鋳型 ,  量 ,  保証