Art

J-GLOBAL ID：202002236936070485   Reference number：20A2491799

Thermostable Mutants of Glycoside Hydrolase Family 6 Cellobiohydrolase from the Basidiomycete Phanerochaete chrysosporium

担子菌Phanerochaete chrysosporium由来のグリコシドヒドロラーゼファミリー6に属するセロビオヒドロラーゼの熱安定性変異体

Author (8)： Yamaguchi Sora (Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo) ,  Sunagawa Naoki (Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo) ,  Tachioka Mikako (Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo) ,  Tachioka Mikako (Deep-Sea Nanoscience Research Group, Research Center for Bioscience and Nanoscience, Japan Agency for Marine-Earth Science and Technology) ,  Igarashi Kiyohiko (Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo) ,  Igarashi Kiyohiko (VTT Technical Research Centre of Finland) ,  Samejima Masahiro (Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo) ,  Samejima Masahiro (Faculty of Engineering, Shinshu University)
Material： Journal of Applied Glycoscience  (応用糖質科学)
Volume： 67  Issue： 3  Page： 79-86(J-STAGE)  Publication year： 2020 
JST Material Number： F0614A  ISSN： 1344-7882  CODEN： JAGLFX  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： Phanerochaete ,...
Semi thesaurus term： Phanerochaete chrysosporium ,...

Register(free) or Log In to access     To see more with JDream III (charged).   {{ this.onShowAbsJLink("http://jdream3.com/lp/jglobal/index.html?docNo=20A2491799&from=J-GLOBAL&jstjournalNo=F0614A") }}

Author keywords (5)： cellobiohydrolase ,  random mutagenesis ,  protein engineering ,  glycoside hydrolase ,  enzymatic saccharification

JST classification (2)： Enzyme in general  (EB09010D) ,  Applications of enzymes  (EB09100B)

Reference (22)：

• 1) S.P. Voutilainen, T. Puranen, M. Siika-Aho, A. Lappalainen, M. Alapuranen, J. Kallio, S. Hooman, L. Viikri, J. Vehmaanperä, and A. Koivula: Cloning, expression, and characterization of novel thermostable family 7 cellobiohydrolases. Biotechnol. Bioeng., 101, 515-528 (2008).
• 2) V. Lombard, H. Golaconda Ramulu, E. Drula, P.M. Coutinho, and B. Henrissat: The carbohydrate-active enzymes database (CAZy) in 2013. Nucleic Acids Res., 42, 490-495 (2014).
• 3) K. Igarashi, T. Uchihashi, A. Koivula, M. Wada, S. Kimura, T. Okamoto, M. Penttilä, T. Ando, and M. Samejima: Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface. Science, 333, 1279-1282 (2011).
• 4) I. Wu and F.H. Arnold: Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperatures. Biotechnol. Bioeng., 110, 1874-1883 (2013).
• 5) X.J. Wang, Y.J. Peng, L.Q. Zhang, A.N. Li, and D.C. Li: Directed evolution and structural prediction of cellobiohydrolase II from the thermophilic fungus Chaetomium thermophilum. Appl. Microbiol. Biotechnol., 95, 1469-1478 (2012).

Terms in the title (8)： 担子菌 ,  Phanerochaete ,  Chrysosporium ,  グリコシドヒドロラーゼ ,  ファミリー ,  セロビオヒドロラーゼ ,  熱安定性 ,  変異体