Art

J-GLOBAL ID：202002290250993674   Reference number：20A2435172

Incorporation of Gelatin Microspheres into HepG2 Human Hepatocyte Spheroids for Functional Improvement through Improved Oxygen Supply to Spheroid Core

スフェロイドコアへの酸素供給改善を介した機能改善のためのHepG2ヒト肝細胞スフェロイドへのゼラチンマイクロスフェアの取り込み

Author (8)： Mizukami Yuya (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Moriya Ai (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Takahashi Yuki (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Shimizu Kazunori (Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University) ,  Konishi Satoshi (Department of Mechanical Engineering, Graduate School of Science and Engineering, Ritsumeikan University) ,  Takakura Yoshinobu (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Nishikawa Makiya (Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University) ,  Nishikawa Makiya (Laboratory of Biopharmaceutics, Faculty of Pharmaceutical Sciences, Tokyo University of Science)
Material： Biological and Pharmaceutical Bulletin (Web)
Volume： 43  Issue： 8  Page： 1220-1225(J-STAGE)  Publication year： 2020 
JST Material Number： U1402A  ISSN： 1347-5215  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *HepG2 cell ,...
Semi thesaurus term： cell physiology ,...

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Author keywords (8)： spheroid ,  gelatin microsphere ,  CYP1A1 ,  oxygen supply ,  spheroid ,  gelatin microsphere ,  CYP1A1 ,  oxygen supply

JST classification (2)： Cell physiology in general  (EF02010S) ,  Cell and tissue culture methods  (EA03040R)

Reference (30)：

• 1) Ong CS, Zhou X, Han J, Huang CY, Nashed A, Khatri S, Mattson G, Fukunishi T, Zhang H, Hibino N. In vivo therapeutic applications of cell spheroids. Biotechnol. Adv., 36, 494-505 (2018).
• 2) Sakai Y, Yamagami S, Nakazawa K. Comparative analysis of gene expression in rat liver tissue and monolayer- and spheroid-cultured hepatocytes. Cells Tissues Organs, 191, 281-288 (2010).
• 3) Nishikawa T, Tanaka Y, Nishikawa M, Ogino Y, Kusamori K, Mizuno N, Mizukami Y, Shimizu K, Konishi S, Takahashi Y, Takakura Y. Optimization of albumin secretion and metabolic activity of cytochrome P450 1A1 of human hepatoblastoma HepG2 cells in multicellular spheroids by controlling spheroid size. Biol. Pharm. Bull., 40, 334-338 (2017).
• 4) Kyffin JA, Sharma P, Leedale J, Colley HE, Murdoch C, Harding AL, Mistry P, Webb SD. Characterisation of a functional rat hepatocyte spheroid model. Toxicol. In Vitro, 55, 160-172 (2019).
• 5) Underhill GH, Khetani SR. Bioengineered liver models for drug testing and cell differentiation studies. Cell. Mol. Gastroenterol. Hepatol., 5, 426-439.e1 (2018).

Terms in the title (7)： 酸素供給 ,  HepG2 ,  ヒト肝細胞 ,  スフェロイド ,  ゼラチン ,  マイクロスフェア ,  取り込み