Art

J-GLOBAL ID：202102210159808970   Reference number：21A0291290

Standardization of an LNA-based TaqMan assay qPCR analysis for Aspiculuris tetraptera DNA in mouse faeces

マウス糞便中のAspiculurisテトラpteraDNAに対するLNAベースTaqManアッセイqPCR分析の標準化【JST・京大機械翻訳】

Author (5)： Isayama Keishiro (Institute of Laboratory Animals, Yamaguchi University Science Research Center, Yamaguchi, Japan) ,  Watanabe Kenji (Institute of Gene Research, Yamaguchi University Science Research Center, Yamaguchi, Japan) ,  Okamoto Mariko (Laboratory of Veterinary Immunology, School of Veterinary Medicine, Azabu University, Sagamihara, Japan) ,  Murata Tomoaki (Institute of Laboratory Animals, Yamaguchi University Science Research Center, Yamaguchi, Japan) ,  Mizukami Yoichi (Institute of Gene Research, Yamaguchi University Science Research Center, Yamaguchi, Japan)
Material： BMC Microbiology (Web)
Volume： 20  Issue： 1  Page： 1-12  Publication year： 2020 
JST Material Number： U7367A  ISSN： 1471-2180  Document type： Article
Article type： 原著論文  Country of issue： United Kingdom (GBR)  Language： ENGLISH (EN)

Abstract/Point： Aspiculuris tetraptera, as a p...

Register(free) or Log In to access     To see more with JDream III (charged).   {{ this.onShowAbsJLink("http://jdream3.com/lp/jglobal/index.html?docNo=21A0291290&from=J-GLOBAL&jstjournalNo=U7367A") }}

Thesaurus term： probe ,  *standardization ,  precipitation (sedimentation) ,  *DNA ,  Microscopy ,  linearity ,  high sensitivity ,  normal phase ,  copy number ,  polymerase chain reaction ,  *quantitative polymerase chain reaction ,  detectability ,  oligodeoxynucleotide ,  genomic DNA ,  *modification ,  aliphatic alcohol

Author keywords (6)： Locked nucleic acid ,  TaqMan assay ,  A. tetraptera ,  Mice ,  PCR ,  Faeces

JST classification (1)： Microorganism test methods  (EG02020G)

Substance index (1)： Ethanol

Reference (30)：

• J Am Assoc Lab Anim Sci; Interspecies variation in the susceptibility of a wild-derived colony of mice to pinworms (Aspiculuris tetraptera); RC Curtis, JK Murray, P Campbell, Y Nagamori, A Molnar, TA Jackson; 56; 1; 2017; 42-46; citation_id=CR1
• Lab Anim; Pinworm infections in laboratory rodents: a review; LF Taffs; 10; 1; 1976; 1-13; 10.1258/002367776780948862; citation_id=CR2
• J Am Assoc Lab Anim Sci; False-positive results after environmental pinworm PCR testing due to Rhabditid nematodes in corncob bedding; M Leblanc, K Berry, S Graciano, B Becker, JD Reuter; 53; 6; 2014; 717-724; citation_id=CR3
• J Am Assoc Lab Anim Sci; Evaluation of traditional and contemporary methods for detecting Syphacia obvelata and Aspiculuris tetraptera in laboratory mice; PM Gerwin, RJ Ricart Arbona, ER Riedel, ML Lepherd, KS Henderson, NS Lipman; 56; 1; 2017; 32-41; citation_id=CR4
• Vet Parasitol; Characterization of rDNA sequences from Syphacia obvelata, Syphacia muris, and Aspiculuris tetraptera and development of a PCR-based method for identification; JD Parel, JU Galula, HK Ooi; 153; 3-4; 2008; 379-383; 10.1016/j.vetpar.2008.02.001; citation_id=CR5

Terms in the title (8)： マウス ,  糞便 ,  テトラ ,  LNA ,  アッセイ ,  qPCR ,  分析 ,  標準化