2023 - 2025 Optical analysis of the heat production mechanism in the brain and its metabolic function in mammalian hibernation.
2020 - 2025 Creation of Neurophotonics and Elucidation of Brain Functions
2023 - 2024 A new role for glucose-sensing neurons under torpor and hibernation
2021 - 2024 A New Role for Glucose-sensing Neurons during Torpor
2020 - 2023 Super-resolution visualization analysis of neural circuit function utilizing nonlinear optical laser technology
2016 - 2021 Advanced Bioimaging Support
2015 - 2020 Resonance Biology for Innovative Bioimaging
2015 - 2020 Sophistication and application of in vivo imaging technologies by using vector laser light
2016 - 2019 Development of super-resolution dark-spot imaging by manipulation of all parameters of light
2016 - 2018 High-speed super-resolution microscopy to visualize living lipid microdomain
2014 - 2017 in vivo super-resolution imaging by utilizing novel laser technologies
2010 - 2016 Multi-dimensional fluorescent live imaging of cellular functions and molecular activities
2010 - 2016 生物個体用in vivo2光子顕微鏡の高度化
2013 - 2015 Improvement of multi-photon excitation microscopy for controlling single cell cAMP level in deep tissue
2010 - 2015 Development and application of in vivo two-photon microscopy for imaging and stimulation
2008 - 2014 ベクトルビームを用いたナノイメージング
2010 - 2012 Two-photon live image rendering - volume exploration of micro biological structure
2010 - 2012 Visualization analysis of neurotransmission and exocytosis by using novel fluorescent protein and laser optics technology
2005 - 2009 Visualization and analysis of membrane fusion mechanism during vesicular transport of transportosome by using multi-photon excitation process
2007 - 2008 Analysis of Calcium-dependent cellular functions by using photoactivation with two-photon microscopy
2007 - 2008 発生の左右性決定に関わるカルシウムシグナルの解明
2006 - 2008 Studies on the role of PRIP in insulin secretion
2007 - 2007 多光子励起レーザー顕微鏡を用いた-分子蛍光in vivoイメージング
2005 - 2006 Cross-sectional image analysis of calcium dependent exocytosis in neurosecretion cell models studied by two-photon microscopy
2000 - 2004 Mechanisms of secretion studied with two-photon excitation imaging and uncaging
2002 - 2002 2光子励起ケイジドグルタミン酸を用いた樹状突起スパイン機能の研究
2001 - 2002 2光子励起法を用いた外分泌腺におけるイオン・水輸送機能の解析
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Papers (149):
Takashi Murata, Motosuke Tsutsumi, Kohei Otomo, Tomomi Nemoto. Cell Division Imaging of Tobacco BY-2 Cells in a 3D Volume by Two-Photon Spinning-Disk Confocal Microscopy. Methods in Molecular Biology. 2024. 37-50
Yuki Wada, Kwangkyun Jang, Hirokazu Ishii, Yuki Watakabe, Motosuke Tsutsumi, Makoto Sako, Tsunayoshi Takehara, Takeyuki Suzuki, Hirofumi Tsujino, Yasuo Tsutsumi, et al. Absorption, Fluorescence, and Two-Photon Excitation Ability of 5-o-Tolyl-11 (or 13)-o-tolylisoindolo[2,1-a]quinolines Prepared by Ring-Closing Metathesis and [2+3] Cycloaddition. Chemistry, an Asian journal. 2024. e202401073
Motosuke Tsutsumi, Kazushi Yamaguchi, Tomomi Nemoto. 4D Evaluation Approach for Cell Signaling Activity in Multicellular Tumor Spheroids. Nanomedicine and Nanotoxicology. 2024. 293-307
Kaito Nakata, Joe Sakamoto, Kohei Otomo, Masanao Sato, Hirokazu Ishii, Motosuke Tsutsumi, Ryosuke Enoki, Tomomi Nemoto. Amyloid-β-induced Alteration of Fast and Localized Calcium Elevations in Cultured Astrocytes. 2024
Kohei Otomo, Takaki Omura, Yuki Nozawa, Steven J Edwards, Yukihiko Sato, Yuri Saito, Shigehiro Yagishita, Hitoshi Uchida, Yuki Watakabe, Kiyotada Naitou, et al. descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques. Nature communications. 2024. 15. 1. 4941-4941
高橋泰伽, 高橋泰伽, 高橋泰伽, 堤香琳, 曽我公平, 曽我公平, 榎木亮介, 岡村陽介, 岡村陽介, 岡村陽介, et al. Large-scale and deep in vivo imaging of the mouse brain utilizing nanomaterials. バイオイメージング. 2024. 33. 2
堤香琳, 榎木亮介, 曽我公平, 曽我公平, 根本知己, 高橋泰伽, 高橋泰伽, 高橋泰伽. In vivo two-photon imaging of dendritic spines at hippocampal CA1 region in hibernation-like state mice. バイオイメージング. 2024. 33. 2
A Cascade Of CNN and LSTM Network With 3D Anchors For Mitotic Cell Detection
(The 44th International Conference on Acoustics, Speech, and Signal Processing, ICASSP 2019 2019)
Four-color three-dimensional live cell imaging by two-photon excitation spinning disk confocal microscopy
(Focus On Microscopy 2019 2019)
Compact two-photon excitation STED nanoscopy with high-peak-power sub-nanosecond 655-nm pulsed light source
(Focus On Microscopy 2019 2019)
In vivo Ca2+ imaging of mouse brain by two-photon excitation spinning-disk confocal microscopy
(9th FOPS congress 2019)
Novel fluoropolymer nanosheets extending in vivo two-photon imaging of living mouse brain
(9th FOPS congress 2019)
2018/12/07 - the 12th International Symposium of Nanomedicine (ISNM2018) The Best Poster Award Novel fluoropolymer nanosheets “PEO-CYTOP” improving open skull window for in vivo imaging of living mouse brain
2018/10/30 - MEXT Grant-in-Aid for Scientific Research on Innovative Areas "Resonance_Bio" International Meeting on Bioimaging for Young Researchers -Chanpuru poster award In vivo two-photon laser ablation within deep regions of living mouse brains
2017/12/14 - International Symposium on Nanomedicine 2017 (ISNM2017) The Best Poster Award In vivo two-photon imaging of living mouse brain by utilizing novel fluoropolymer PEO-CYTOP nanosheets
2017/09/17 - 第26回日本バイオイメージング学会学術集会 ベストイメージング賞ニコン賞 In vivo イメージングの改善のための最適な観察条件の探索
2017/09/17 - 第26回日本バイオイメージング学会学術集会 ベストイメージング賞ツァイス賞 多点走査型2光子顕微鏡を用いたマウス膵臓におけるin vivo Ca2+イメージング
2017/03/14 - 第二回北大部局横断シンポジウム ベストポスター賞 in vivo 2光子顕微鏡法を用いたマウス生体脳深部における単一神経線維破断
2017/02/13 - 北海道大学工学部情報エレクトロニクス学科 生体情報工学学生研究賞
2017/02/13 - 北海道大学大学院情報科学研究科 吉本賞
2017/02/13 - 北海道大学大学院情報科学研究科 三上賞
2016/09/23 - 日本学術振興会 平成28年度「科研費」審査委員受賞
2016/03 - 日本生理学会 学生ポスター賞 In vivo two-photon laser ablation by improvement of focusing property in living mouse brains
2015/11 - The 16 th RIES-Hokudai international symposium 2015 Excellent Poster Award 3D-visualization of SVZ-derived immature neurons in postnatal mouse brain for the elucidation of migratory behavior”
2015/04 - Analytical Sciences Hot Article Award Multi-point scanning two-photon excitation microscopy utilising a high-peak-power 1042-nm laser
2015/02 - 財団法人北海道大学クラーク記念財団 クラーク賞 In-vivo nerve fiber severance within deeper regions in living mouse brain by near-infrared ultra-short laser light pulse
2014/12 - the 15 th RIES-Hokudai international symposium 2014 優秀ポスター賞 Evaluation of in vivo two-photon microscopy by imaging of fluorescent beads in mouse brain
日本ナノメデイシン交流協会
, 国際複合医工学会
, BIOIMAGING SOCIETY
, THE SPECTROSCOPICAL SOCIETY OF JAPAN
, THE MOLECULAR BIOLOGY SOCIETY OF JAPAN
, THE PHYSIOLOGICAL SOCIETY OF JAPAN
, THE BIOPHYSICAL SOCIETY OF JAPAN
, 日本顕微鏡学会