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J-GLOBAL ID：200902232766780075   Reference number：05A0143530

Transient RNA Silencing of Scoulerine 9-O-Methyltransferase Expression by Double Stranded RNA in Coptis japonica Protoplasts

Coptis japonicaのプロトプラストにおける二本鎖RNAによるスコウレリン9-O-メチルトランスフェラーゼの一過性RNAサイレンシング

Author (7)： DUBOUZET J G (Kyoto Univ., Kyoto, JPN) ,  MORISHIGE T (Kyoto Univ., Kyoto, JPN) ,  FUJII N (Kyoto Univ., Kyoto, JPN) ,  AN C-I (Osaka Univ., Osaka, JPN) ,  FUKUSAKI E (Osaka Univ., Osaka, JPN) ,  IFUKU K (Kyoto Univ., Kyoto, JPN) ,  SATO F (Kyoto Univ., Kyoto, JPN)
Material： Bioscience, Biotechnology, and Biochemistry  (Bioscience Biotechnology & Biochemistry)
Volume： 69  Issue： 1  Page： 63-70  Publication year： Jan. 23, 2005 
JST Material Number： G0021A  ISSN： 0916-8451  CODEN： BBBIEJ  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *methyltransferase ,...
Semi thesaurus term： Coptis japonica

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JST classification (2)： Enzyme physiology  (EH02020J) ,  Gene expression  (EC02040Q)

Reference (24)：

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• 2) Fiehn, O., Metabolomics: the link between genotypes and phenotypes. Plant Mol. Biol., 48, 155–171 (2002).
• 3) Ikezawa, N., Tanaka, M., Nagayoshi, M., Shinkyo, R., Sakaki, T., Inouye, K., and Sato, F., Molecular cloning and characterization of CYP719, a methylenedioxy bridge-forming enzyme that belongs to a novel P450 family, from cultured Coptis japonica cells. J. Biol. Chem., 278, 38557–38565 (2003).
• 4) Sato, F., and Yamada, Y., Berberine-producing cultures of Coptis japonica cells. Phytochemistry, 23, 281–285 (1984).
• 5) Choi, K. B., Morishige, T., Shitan, N., Yazaki, K., and Sato, F., Molecular cloning and characterization of coclaurine N-methyltransferase from cultured cells of Coptis japonica. J. Biol. Chem., 277, 830–835 (2002).

Terms in the title (4)： プロトプラスト ,  二本鎖RNA ,  O-メチルトランスフェラーゼ ,  RNAサイレンシング