Art

J-GLOBAL ID：200902295436902575   Reference number：07A1047205

High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells: Rapid Purification and Characterization

Sf-9細胞における活性な組換え体ヒトレニンの高レベル発現: 迅速な精製および特性づけ

Author (4)： TAKAHASHI Saori (Inst. for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Res. Center) ,  HATA Keishi (Inst. for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Res. Center) ,  KIKUCHI Ken-ichi (Dep. of Materials-Process Engineering and Applied Chemistry for the Environment, Akita Univ.) ,  GOTOH Takeshi (Dep. of Materials-Process Engineering and Applied Chemistry for the Environment, Akita Univ.)
Material： Bioscience, Biotechnology, and Biochemistry  (Bioscience Biotechnology & Biochemistry)
Volume： 71  Issue： 10  Page： 2610-2613 (J-STAGE)  Publication year： 2007 
JST Material Number： G0021A  ISSN： 0916-8451  CODEN： BBBIEJ  Document type： Article
Article type： 短報  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *Renin ,...
Semi thesaurus term： 組換体酵素

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JST classification (2)： Enzyme in general  (EB09010D) ,  Substance production by animal tissues and cells  (FK03060S)

Reference (19)：

• 1) Takahashi, S., Ohsawa, T., Miura, R., and Miyake, Y., Purification of high molecular weight (MHW) renin from porcine kidney and direct evidence that HMW renin is a complex of renin with renin binding protein (RnBP). J. Biochem., 93, 265–274 (1983).
• 2) Inoue, H., Fukui, K., Takahashi, S., and Miyake, Y., Molecular cloning and sequence analysis of a cDNA encoding a porcine kidney renin-binding protein. J. Biol. Chem., 265, 6556–6561 (1990).
• 3) Takahashi, S., Inoue, H., Fukui, K., and Miyake, Y., Structure and function of renin binding protein. Kidney Int., 46, 1525–1527 (1994).
• 4) Imai, T., Cho, T., Takamatsu, H., Hori, H., Saito, M., Masuda, T., Hirose, S., and Murakami, K., Synthesis and characterization of human prorenin in Escherichia coli. J. Biochem., 100, 425–432 (1986).
• 5) Takahashi, S., Ogasawara, H., Watanabe, T., Kumagai, M., Inoue, H., and Hori, K., Refolding and activation of human prorenin expressed in Escherichia coli: application of recombinant human renin for inhibitor screening. Biosci. Biotechnol. Biochem., 70, 2913–2918 (2006).

Terms in the title (8)： SF ,  細胞 ,  活性 ,  組換え体 ,  高レベル ,  発現 ,  精製 ,  特性づけ