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J-GLOBAL ID：200902233385815936   Reference number：07A0009037

Refolding and Activation of Human Prorenin Expressed in Escherichia coli: Application of Recombinant Human Renin for Inhibitor Screening

Escherichia coliで発現したヒトプロレニンのリフォールディングおよび活性化:阻害剤スクリーニングのための組換体ヒトレニンの適用

Author (6)： TAKAHASHI Saori (Akita Res. Inst. for Food and Brewing) ,  OGASAWARA Hironobu (Akita Res. Inst. for Food and Brewing) ,  WATANABE Takayuki (Akita Res. Inst. for Food and Brewing) ,  KUMAGAI Masanori (Akita Res. Inst. for Food and Brewing) ,  INOUE Hiroyasu (Dep. of Food Sci. and Nutrition, Fac. of Human Life and Environment, Nara Women’s Univ.) ,  HORI Kazuyuki (Akita Res. Inst. for Food and Brewing)
Material： Bioscience, Biotechnology, and Biochemistry  (Bioscience Biotechnology & Biochemistry)
Volume： 70  Issue： 12  Page： 2913-2918 (J-STAGE)  Publication year： 2006 
JST Material Number： G0021A  ISSN： 0916-8451  CODEN： BBBIEJ  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *renin ,...
Semi thesaurus term： prorenin ,...

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JST classification (2)： Vegetables and processed vegetable products  (FJ11020O) ,  Enzyme in general  (EB09010D)

Reference (40)：

• 1) Freeman, R. H., and Davis, J. O., Factors controlling renin secretion and metabolism. In “Hypertension, Physiopathology and Treatment, 2nd Edition,” eds. Genest, J., Kuchel, O., Hamet, P., and Cantin, M., McGraw-Hill Inc., New York, pp. 225–250 (1983).
• 2) Takahashi, S., Ohsawa, T., Miura, R., and Miyake, Y., Purification and characterization of renin binding protein (RnBP) from porcine kidney. J. Biochem., 93, 1583–1594 (1983).
• 3) Inoue, H., Fukui, K., Takahashi, S., and Miyake, Y., Molecular cloning and sequence analysis of a cDNA encoding a porcine kidney renin-binding protein. J. Biol. Chem., 265, 6556–6561 (1990).
• 4) Takahashi, S., Inoue, H., Fukui, K., and Miyake, Y., Structure and function of renin binding protein. Kidney Int., 46, 1525–1527 (1994).
• 5) Takahashi, S., Takahashi, K., Kaneko, T., Ogasawara, H., Shindo, S., and Kobayashi, M., Human renin binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase. J. Biochem., 125, 348–353 (1999).

Terms in the title (7)： Escherichia ,  発現 ,  リフォールディング ,  活性化 ,  阻害剤 ,  スクリーニング ,  組換体