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J-GLOBAL ID：201202281151264570   Reference number：12A0693661

Efficient expression and purification of recombinant human m-calpain using an Escherichia coli expression system at low temperature

大腸菌発現系を用いて低温での組換ヒトm-カルパインの効率的発現及び精製

Author (4)： HATA Shoji (Tokyo Metropolitan Inst. Medical Sci., Tokyo, JPN) ,  UENO Mika (Tokyo Metropolitan Inst. Medical Sci., Tokyo, JPN) ,  KITAMURA Fujiko (Tokyo Metropolitan Inst. Medical Sci., Tokyo, JPN) ,  SORIMACHI Hiroyuki (Tokyo Metropolitan Inst. Medical Sci., Tokyo, JPN)
Material： Journal of Biochemistry  (JB)
Volume： 151  Issue： 4  Page： 417-422  Publication year： Apr. 2012 
JST Material Number： W1920A  ISSN： 0021-924X  CODEN： JOBIAO  Document type： Article
Article type： 原著論文  Country of issue： United Kingdom (GBR)  Language： ENGLISH (EN)

Thesaurus term： *Calpain ,...

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JST classification (3)： Enzyme in general  (EB09010D) ,  Gene manipulation  (EC02050B) ,  Animal biochemistry  (EJ01050P)

Reference (14)：

• GOLL, D. E. The calpain system. Physiol. Rev. 2003, 83, 731-801
• SORIMACHI, H. Impact of genetic insights into calpain biology. J. Biochem. 2011, 150, 23-37
• STROBL, S. The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium. Proc. Natl. Acad. Sci. USA. 2000, 97, 588-592
• HOSFIELD, C. M. Crystal structure of calpain reveals the structural basis for Ca(^<2+>)-dependent protease activity and a novel mode of enzyme activation. EMBO J. 1999, 18, 6880-6889
• MOLDOVEANU, T. Concerted multi-pronged attack by calpastatin to occlude the catalytic cleft of heterodimeric calpains. Nature. 2008, 456, 404-408

Terms in the title (7)： 大腸菌 ,  発現 ,  低温 ,  組換 ,  ヒト ,  m-カルパイン ,  精製