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J-GLOBAL ID：201402207951150326   Reference number：14A0604045

Design and Experimental Application of a Novel Non-Degenerate Universal Primer Set that Amplifies Prokaryotic 16S rRNA Genes with a Low Possibility to Amplify Eukaryotic rRNA Genes

真核生物rRNA遺伝子を増幅する可能性は低いが,原核生物16S rRNA遺伝子を増幅する新しい非縮重ユニバーサルプライマーセットの設計及び実験的適用

Author (12)： MORI Hiroshi (Tokyo Inst. Technol., Yokohama, JPN) ,  MORI Hiroshi (Tokyo Inst. Technol., Tokyo, JPN) ,  MARUYAMA Fumito (Tokyo Medical and Dental Univ., Tokyo, JPN) ,  KATO Hiromi (Tohoku Univ., Sendai, JPN) ,  TOYODA Atsushi (National Inst. Genetics, Shizuoka, JPN) ,  DOZONO Ayumi (Tokyo Inst. Technol., Yokohama, JPN) ,  OHTSUBO Yoshiyuki (Tohoku Univ., Sendai, JPN) ,  NAGATA Yuji (Tohoku Univ., Sendai, JPN) ,  FUJIYAMA Asao (National Inst. Genetics, Shizuoka, JPN) ,  FUJIYAMA Asao (National Inst. Informatics, Tokyo, JPN) ,  TSUDA Masataka (Tohoku Univ., Sendai, JPN) ,  KUROKAWA Ken (Tokyo Inst. Technol., Tokyo, JPN)
Material： DNA Research  (DNA Res)
Volume： 21  Issue： 2  Page： 217-227  Publication year： Apr. 2014 
JST Material Number： W1628A  ISSN： 1340-2838  Document type： Article
Article type： 原著論文  Country of issue： United Kingdom (GBR)  Language： ENGLISH (EN)

Thesaurus term： *procaryote ,...
Semi thesaurus term： *16S rRNA gene ,...

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JST classification (2)： Research methods in genetics  (EC01020N) ,  Microorganism morphology and taxonomy  (EG03030Y)

Reference (42)：

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• Hamady, M.,Walker, J.J., Harris, J.K.,Gold, N.J.andKnight, R. 2008, Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex, Nat. Methods, 5, 235-7.
• Mori, H.,Maruyama, F.andKurokawa, K.2010,VITCOMIC: visualization tool for taxonomic compositions of microbial communities based on 16S rRNA gene sequences, BMC Bioinformatics, 11, 332.
• Turnbaugh, P.J., Hamady, M., Yatsunenko, T., et al. 2009, A core gut microbiome in obese and lean twins, Nature, 457, 480-4.

Terms in the title (8)： 真核生物 ,  rRNA遺伝子 ,  増幅 ,  可能性 ,  原核生物 ,  縮重 ,  設計 ,  実験