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J-GLOBAL ID：201702274467453764   Reference number：17A1973913

Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes

ヒト人工多能性幹細胞と分化した心筋細胞のための効率的な大規模2D培養システム【Powered by NICT】

Author (22)： Tohyama Shugo (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Tohyama Shugo (Department of Organ Fabrication, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Fujita Jun (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Fujita Chihana (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Yamaguchi Miho (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Kanaami Sayaka (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Ohno Rei (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Sakamoto Kazuho (Department of Pharmacology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan) ,  Sakamoto Kazuho (Department of Bio-Informational Pharmacology, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan) ,  Kodama Masami (Department of Bio-informational Pharmacology, Medical Research Institute, National University Corporation Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan) ,  Kurokawa Junko (Department of Bio-Informational Pharmacology, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan) ,  Kanazawa Hideaki (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Seki Tomohisa (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Kishino Yoshikazu (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Okada Marina (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Nakajima Kazuaki (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Tanosaki Sho (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Someya Shota (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Hirano Akinori (Department of Cardiovascular Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Kawaguchi Shinji (Department of Cardiovascular Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Kobayashi Eiji (Department of Organ Fabrication, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan) ,  Fukuda Keiichi (Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan)
Material： Stem Cell Reports
Volume： 9  Issue： 5  Page： 1406-1414  Publication year： 2017 
JST Material Number： W3132A  ISSN： 2213-6711  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Abstract/Point： Cardiac regenerative therapies...

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Thesaurus term： Heart ,  *Cardiac Myocyte ,  *culture (biology) ,  Metabolism ,  *versatility ,  *differentiation (biology) ,  *human ,  protocol ,  multilayer ,  *induced pluripotent stem cell ,  two dimension ,  aliphatic alcohol ,  aliphatic carboxylic acid ,  secondary alcohol ,  hydroxy acid ,  amino acid ,  aliphatic amine ,  carboxamide ,  dicarboxylic acid ,  primary amine ,  hexose ,  aldose
Semi thesaurus term： 心臓再生 , 【Automatic Indexing@JST】

Author keywords (6)： iPSCs ,  regenerative medicine ,  cardiomyocytes ,  purification ,  large scale ,  2D culture

JST classification (2)： Cell physiology in general  (EF02010S) ,  Cell and tissue culture methods  (EA03040R)

Substance index (3)： Lactic acid ,  Glutamine ,  Glucose

Terms in the title (7)： ヒト ,  人工多能性幹細胞 ,  分化 ,  心筋細胞 ,  大規模 ,  2D ,  培養