Art

J-GLOBAL ID：201702286893592652   Reference number：17A0216452

Efficient pig ICSI using Percoll-selected spermatozoa; evidence for the essential role of phospholipase C-ζ in ICSI success

パーコール選抜精子を用いる効率的なブタICSI:ICSIの成功におけるホスホリパーゼCζの重要な役割に関する証拠

Author (9)： NAKAI Michiko (Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Ibaraki 305-8602, Japan) ,  SUZUKI Shun-ichi (Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Ibaraki 305-8602, Japan) ,  ITO Junya (Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Kanagawa 252-5201, Japan) ,  FUCHIMOTO Dai-ichiro (Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Ibaraki 305-8602, Japan) ,  SEMBON Shoichiro (Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Ibaraki 305-8602, Japan) ,  NOGUCHI Junko (Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Ibaraki 305-8602, Japan) ,  ONISHI Akira (College of Bioresource Sciences, Nihon University, Kanagawa 252-0880, Japan) ,  KASHIWAZAKI Naomi (Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Kanagawa 252-5201, Japan) ,  KIKUCHI Kazuhiro (Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Ibaraki 305-8602, Japan)
Material： Journal of Reproduction and Development  (J Reprod Dev)
Volume： 62  Issue： 6  Page： 639-643(J-STAGE)  Publication year： 2016 
JST Material Number： F0766A  ISSN： 0916-8818  Document type： Article
Article type： 短報  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *Sus scrofa domesticus ,...
Semi thesaurus term： *ICSI ,...

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Author keywords (4)： Fertilization ,  Intracytoplasmic sperm injection ,  Oocyte activation ,  Pig

JST classification (3)： Swine(=pigs)  (FD02040U) ,  Reproduction management  (FD01030C) ,  Reproductive(=genital)organs  (EJ15020P)

Reference (27)：

• 1. Saunders CM, Larman MG, Parrington J, Cox LJ, Royse J, Blayney LM, Swann K, Lai FA. PLC ζ: a sperm-specific trigger of Ca²⁺ oscillations in eggs and embryo development. Development 2002; 129: 3533-3544.
• 2. Schultz RM, Kopf GS. Molecular basis of mammalian egg activation. Curr Top Dev Biol 1995; 30: 21-62.
• 3. Lee JW, Tian XC, Yang X. Failure of male pronucleus formation is the major cause of lack of fertilization and embryo development in pig oocytes subjected to intracytoplasmic sperm injection. Biol Reprod 2003; 68: 1341-1347.
• 4. Nakai M, Kashiwazaki N, Takizawa A, Maedomari N, Ozawa M, Noguchi J, Kaneko H, Shino M, Kikuchi K. Morphologic changes in boar sperm nuclei with reduced disulfide bonds in electrostimulated porcine oocytes. Reproduction 2006; 131: 603-611.
• 5. Lai L, Sun Q, Wu G, Murphy CN, Kühholzer B, Park KW, Bonk AJ, Day BN, Prather RS. Development of porcine embryos and offspring after intracytoplasmic sperm injection with liposome transfected or non-transfected sperm into in vitro matured oocytes. Zygote 2001; 9: 339-346.

Terms in the title (8)： コール ,  精子 ,  ブタ ,  ICSI ,  成功 ,  ホスホリパーゼCζ ,  役割 ,  証拠