文献

J-GLOBAL ID：201902286470864422   整理番号：19A2336656

条件付きマウス対立遺伝子の生成のためのCRISPR-Cas9法の再現性:多施設評価【JST・京大機械翻訳】

Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

著者 (105件)： Gurumurthy Channabasavaiah B. (Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, USA) ,  Gurumurthy Channabasavaiah B. (Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, USA) ,  O’Brien Aidan R. (Transformational Bioinformatics, Health and Biosecurity Business Unit, CSIRO, North Ryde, Australia) ,  O’Brien Aidan R. (Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia) ,  Quadros Rolen M. (Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, USA) ,  Adams John (Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, USA) ,  Alcaide Pilar (Department of Immunology, Tufts University School of Medicine, Boston, USA) ,  Ayabe Shinya (RIKEN BioResource Research Center, Tsukuba, Japan) ,  Ballard Johnathan (Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, USA) ,  Batra Surinder K. (Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, USA) ,  Beauchamp Marie-Claude (Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada) ,  Becker Kathleen A. (Maine Medical Center Research Institute (MMCRI), Scarborough, USA) ,  Bernas Guillaume (Transgenesis and Animal Modeling Core Facility, Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada) ,  Brough David (Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK) ,  Carrillo-Salinas Francisco (Department of Immunology, Tufts University School of Medicine, Boston, USA) ,  Chan Wesley (Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada) ,  Chen Hanying (School of Medicine, Indiana University, Indianapolis, USA) ,  Dawson Ruby (South Australian Health & Medical Research Institute and Department of Medicine, University of Adelaide, Adelaide, Australia) ,  DeMambro Victoria (Maine Medical Center Research Institute (MMCRI), Scarborough, USA) ,  D’Hont Jinke (Transgenic Mouse Core Facility, VIB Center for Inflammation Research, Ghent, Belgium) ,  D’Hont Jinke (Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium) ,  Dibb Katharine M. (Unit of Cardiac Physiology, School of Medical Sciences, Manchester Academic Health Science Center, University of Manchester, Manchester, UK) ,  Eudy James D. (High-Throughput DNA Sequencing and Genotyping Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, USA) ,  Gan Lin (University of Rochester Medical Center, Rochester, USA) ,  Gao Jing (Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia) ,  Gonzales Amy (Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, USA) ,  Guntur Anyonya R. (Maine Medical Center Research Institute (MMCRI), Scarborough, USA) ,  Guo Huiping (Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, USA) ,  Harms Donald W. (Mouse Genome Engineering Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, USA) ,  Harrington Anne (Maine Medical Center Research Institute (MMCRI), Scarborough, USA) ,  Hentges Kathryn E. (Division of Evolution and Genomic Sciences, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK) ,  Humphreys Neil (Transgenic Unit Core Facility, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK) ,  Imai Shiho (Department of Basic Medicine, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Isehara, Japan) ,  Ishii Hideshi (Department of Medical Data Science, Osaka University Graduate School of Medicine, Suita, Japan) ,  Iwama Mizuho (RIKEN BioResource Research Center, Tsukuba, Japan) ,  Jonasch Eric (The University of Texas MD Anderson Cancer Center, Houston, USA) ,  Karolak Michelle (Maine Medical Center Research Institute (MMCRI), Scarborough, USA) ,  Keavney Bernard (Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, The University of Manchester and Manchester Heart Centre, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK) ,  Khin Nay-Chi (Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia) ,  Konno Masamitsu (Department of Frontier Science for Cancer and Chemotherapy, Osaka University Graduate School of Medicine, Suita, Japan) ,  Kotani Yuko (The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Japan) ,  Kunihiro Yayoi (The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Japan) ,  Lakshmanan Imayavaramban (Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, USA) ,  Larochelle Catherine (Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada) ,  Lawrence Catherine B. (Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK) ,  Li Lin (Children’s Research Institute Mouse Genome Engineering Core, University of Texas Southwestern Medical Center, Dallas, USA) ,  Lindner Volkhard (Maine Medical Center Research Institute (MMCRI), Scarborough, USA) ,  Liu Xian-De (The University of Texas MD Anderson Cancer Center, Houston, USA) ,  Lopez-Castejon Gloria (Manchester Collaborative Centre for Inflammation Research (MCCIR), School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK) ,  Loudon Andrew (Centre for Biological Timing, School of Medical Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK) ,  Lowe Jenna (Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia) ,  Jerome-Majewska Loydie A. (Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada) ,  Matsusaka Taiji (Department of Basic Medicine, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Isehara, Japan) ,  Miura Hiromi (Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Japan) ,  Miura Hiromi (Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Isehara, Japan) ,  Miyasaka Yoshiki (The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Japan) ,  Morpurgo Benjamin (Texas A&M Institute for Genomic Medicine (TIGM), Texas A&M University, College Station, USA) ,  Motyl Katherine (Maine Medical Center Research Institute (MMCRI), Scarborough, USA) ,  Nabeshima Yo-ichi (Laboratory of Molecular Life Science, Foundation for Biomedical Research and Innovation, Kobe, Japan) ,  Nakade Koji (RIKEN BioResource Research Center, Tsukuba, Japan) ,  Nakashiba Toshiaki (RIKEN BioResource Research Center, Tsukuba, Japan) ,  Nakashima Kenichi (RIKEN BioResource Research Center, Tsukuba, Japan) ,  Obata Yuichi (RIKEN BioResource Research Center, Tsukuba, Japan) ,  Ogiwara Sanae (Department of Laboratory Animal Science, Support Center for Medical Research and Education, Tokai University, Isehara, Japan) ,  Ouellet Mariette (Transgenesis and Animal Modeling Core Facility, Centre de Recherche du Centre Hospitalier Universitaire de Montreal (CRCHUM), Montreal, Canada) ,  Oxburgh Leif (Maine Medical Center Research Institute (MMCRI), Scarborough, USA) ,  Oxburgh Leif (Basic and Clinical Research, The Rogosin Institute, New York, USA) ,  Piltz Sandra (South Australian Health & Medical Research Institute and Department of Medicine, University of Adelaide, Adelaide, Australia) ,  Pinz Ilka (Maine Medical Center Research Institute (MMCRI), Scarborough, USA) ,  Ponnusamy Moorthy P. (Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, USA) ,  Ray David (Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, UK) ,  Redder Ronald J. (High-Throughput DNA Sequencing and Genotyping Core Facility, Vice Chancellor for Research Office, University of Nebraska Medical Center, Omaha, USA) ,  Rosen Clifford J. (Maine Medical Center Research Institute (MMCRI), Scarborough, USA) ,  Ross Nikki (Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia) ,  Ruhe Mark T. (Mouse Biology Program, University of California, Davis, USA) ,  Ryzhova Larisa (Maine Medical Center Research Institute (MMCRI), Scarborough, USA) ,  Salvador Ane M. (Department of Immunology, Tufts University School of Medicine, Boston, USA) ,  Alam Sabrina Shameen (Departments of Anatomy and Cell Biology, Human Genetics and Pediatrics, Research Institute McGill University Health Center (RI-MUHC), Montreal, Canada) ,  Sedlacek Radislav (Laboratory of Transgenic Models of Diseases and Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic) ,  Sharma Karan (College of Osteopathic Medicine, Marian University, Indianapolis, USA) ,  Smith Chad (The University of Texas MD Anderson Cancer Center, Houston, USA) ,  Staes Katrien (Transgenic Mouse Core Facility, VIB Center for Inflammation Research, Ghent, Belgium) ,  Staes Katrien (Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium) ,  Starrs Lora (Department of Immunology and Infectious Disease, The John Curtin School of Medical Research, the Australian National University, Canberra, Australia) ,  Sugiyama Fumihiro (Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Japan) ,  Takahashi Satoru (Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Japan) ,  Tanaka Tomohiro (Department of Gastroenterology and Metabolism, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan) ,  Trafford Andrew W. (Unit of Cardiac Physiology, School of Medical Sciences, Manchester Academic Health Science Center, University of Manchester, Manchester, UK) ,  Uno Yoshihiro (The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Japan) ,  Vanhoutte Leen (Transgenic Mouse Core Facility, VIB Center for Inflammation Research, Ghent, Belgium) ,  Vanhoutte Leen (Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium) ,  Vanrockeghem Frederique (Transgenic Mouse Core Facility, VIB Center for Inflammation Research, Ghent, Belgium) ,  Vanrockeghem Frederique (Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium) ,  Willis Brandon J. (Mouse Biology Program, University of California, Davis, USA) ,  Wright Christian S. (School of Health and Human Sciences, Department of Physical Therapy, Indiana University, Indianapolis, USA) ,  Yamauchi Yuko (The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Japan) ,  Yi Xin (School of Health and Human Sciences, Department of Physical Therapy, Indiana University, Indianapolis, USA) ,  Yoshimi Kazuto (The Institute of Experimental Animal Sciences, Osaka University Graduate School of Medicine, Suita, Japan) ,  Zhang Xuesong (The University of Texas MD Anderson Cancer Center, Houston, USA) ,  Zhang Yu (Children’s Research Institute Mouse Genome Engineering Core, University of Texas Southwestern Medical Center, Dallas, USA) ,  Ohtsuka Masato (Center for Matrix Biology and Medicine, Graduate School of Medicine, Tokai University, Isehara, Japan) ,  Ohtsuka Masato (Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Isehara, Japan) ,  Das Satyabrata (Lillehei Heart Institute Regenerative Medicine and Sciences Program, University of Minnesota, Minneapolis, USA) ,  Garry Daniel J. (Paul and Sheila Wellstone Muscular Dystrophy Center, University of Minnesota, Minneapolis, USA) ,  Garry Daniel J. (Department of Surgery, School of Medicine, University of California, Davis, Davis, USA)
資料名： Genome Biology (Web)  (Genome Biology (Web))
巻： 20  号： 1  ページ： 1-14  発行年： 2019年 
JST資料番号： U7384A  ISSN： 1474-760X  資料種別： 逐次刊行物 (A)
記事区分： 原著論文  発行国： イギリス (GBR)  言語： 英語 (EN)

抄録/ポイント： CRISPR-Cas9遺伝子編集技術はノックアウトマウスの生成を促進し,面倒で時間がかかる従来の胚幹細胞に基づく方法の代替法を提供する。2つの単一ガイドRNA(sgRNA)と2つの一本鎖オリゴヌクレオチドをドナーとしてマイクロインジェクション(sgRNA)と2つの一本鎖オリゴヌクレオチドをドナーとして用いて,条件付きノックアウト(cKOまたはfloxed)対立遺伝子の発生において16%の効率までの以前の研究が報告されている。著者らは,世界中の20の研究所の共同体から2つのドナー方法を再評価した。データセットは,56の遺伝子座,17887の接合子および1718の生きたマウスを構成し,そのうち15(0.87%)のマウスのみがcKO対立遺伝子を含んでいた。データセットを統計解析と機械学習アルゴリズムに従い,解析した因子のどれもこの方法の成功を予測しないことを明らかにした。著者らは,2ドナーアプローチがcKO対立遺伝子を産生できない18の遺伝子座に1ドナーDNAを用いるいくつかの新しい方法を試験した。著者らは,1-ドナー法が2-ドナー法より10~20倍効率的であることを見出した。著者らは,2つのドナー方法が,それがcisにおける2つの同時再結合イベントに依存するので,効率を欠いていることを提案する。それは,望ましくない編集イベントを伴う普及によってdwar与えたされる結果である。1つのドナーDNAを使用する方法は,それらが1つの再結合イベントだけに依存するので,かなり効率的であり,予想されていない突然変異イベントのないドナーカセットの正しい挿入の確率は非常に高い。したがって,1ドナー法は,cKO動物モデルのルーチン発生に対してより高い効率を提供する。Copyright 2019 The Author(s) All rights reserved. Translated from English into Japanese by JST.【JST・京大機械翻訳】

シソーラス用語： 再結合 ,  ES細胞 ,  *対立遺伝子 ,  ノックアウトマウス ,  オリゴヌクレオチド ,  ドナー ,  編集 ,  突然変異 ,  型ばらし ,  *再現性 ,  DNA【核酸】
準シソーラス用語： 動物モデル ,  sgRNA ,  *Cas9 ,  *CRISPR配列 , 【Automatic Indexing@JST】

分類 (3件)： 遺伝子の構造と化学  (EC02020U) ,  遺伝子発現  (EC02040Q) ,  分子遺伝学一般  (EC02010J)

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タイトルに関連する用語 (6件)： マウス ,  対立遺伝子 ,  CRISPR-Cas9法 ,  再現性 ,  施設 ,  評価