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J-GLOBAL ID：200901010260108534 Update date: Mar. 28, 2025

Ando Toshio

アンドウトシオ | Ando Toshio

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Affiliation and department：
Homepage URL (1)： http://biophys.w3.kanazawa-u.ac.jp/

Research field (3)： Biophysics , Nanobioscience , Nanomaterials

Research keywords (8)： Scanning Probe Microscopy , Bio-imaging , High-speed atomic force microscopy , High-speed AFM , atomic force microscopy , motor proteins , Atomic Force Microscope , single-molecule analysis

Research theme for competitive and other funds (42)：

2022 - 2026 Development of high-speed AFM capable of filming at 100 frames/s and its demonstrative studies
2020 - 2024 Self-organization and biomechanical properties of the endosomal membrane
2017 - 2022 Materializing nanoscale dynamic imaging of protein molecules in extremely soft membrane environments
2019 - 2020 「動的構造生命科学を拓く新発想測定技術」の成果取りまとめを目指した組織活動
2015 - 2019 Support activities aimed at international network formation in the research area of dynamic structural life sciences

2014 - 2019 New measurement techniques for visualizing live protein molecules at work

2014 - 2019 Development of advanced high-speed AFM and analysis of proteins' action mechanism

2013 - 2019 Dynamic structural biology of protein machineries driven by ATP/GTP

2012 - 2017 New structural biology to be opened by high-speed atomic force microscopy

2012 - 2016 Development of scanner for probe-scan-type of high-speed AFM

2011 - 2014 Visualizing nanometer- scale structural plasticity of synapses in real time using AFM

2011 - 2014 Structural Basis for the chromatin remodeling by nuclear receptors

2009 - 2014 Development of new methods to determine IDP structure

2008 - 2013 International Consortium Program for High-speed Bio-AFM

2008 - 2013 Development of high-speed microscopy capable of capturing fine structures of live cells

2008 - 2012 Innovative high-speed AFM for elucidating vital phenomena

2004 - 2010 Development of instrument for 3D high-resolution dynamics analysis of biological molecules

2004 - 2010 Development of High-speed Imaging Apparatus for Studying Nanometer Scale Dynamic Behavior of Proteins

2005 - 2010 System Cell Engineering by Multi-scale Manipulation

2003 - 2008 Development of a highest-speed atomic force microscope and elucidation of the nano-structural dynamics of biological Molecular motors

2004 - 2005 アクチンに形成される場で無生物は走るか?

2002 - 2003 タンパク質の分子機能動態及び場のリアルタイムイメージングを目指して

2002 - 2002 シャペロニン機能動態のナノイメージング

2000 - 2002 Mechanochechemical Coupling in Brain Myosin V

2000 - 2000 生物分子モーターの構造・機能の1分子動態解析研究

1999 - 1999 生物分子モーターの構造・機能の1分子動態解析研究

1998 - 1999 Visualization of Hydrophobic-, Hydrophilic-, and Charged Areas of Protein by Photochromic Cantilevers.

1998 - 1998 生物分子モーターの構造・機能の1分子動態解析研究

1996 - 1998 1原子・1分子レベルの解析と操作による細胞膜機能の解明

1997 - 1997 生物分子モーターの構造・機能の1分子動態解析研究.

1997 - 1997 ポリマーの結晶化とゲル相転移の研究

1996 - 1996 蛍光プローブ走査型ナノ顕微鏡によるタンパク質表面物性の研究

1994 - 1996 Manufacture of Scanning Force Microscope for Biological Research

1994 - 1996 Molecular Energetics of Protein Motors

1995 - 1995 ヘテロポリマーの物性とprotein foldingへの応用

1994 - 1994 原子間力顕微鏡によるアクトミオシン系の1分子力学特性の研究

1991 - 1993 Development of an Atomic Force Microscope Suitable for Observation of Biological Samples.

1990 - 1991 DIRECTIONAL BROWNIAN MOTION IN UNIFORM HEAT BATH

1989 - 1991 滑りの分子ダイナミックス

1988 - 1988 骨格筋のアクチン繊維鎖に沿う構造変化の伝播-その非対称性

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Papers (194)：

Muhammad Isman Sandira, Keesiang Lim, Takeshi Yoshida, Elma Sakinatus Sajidah, Shinnosuke Narimatsu, Reon Imakawa, Kota Yoshimura, Goro Nishide, Yujia Qiu, Azuma Taoka, et al. Nanoscopic Profiling of Small Extracellular Vesicles via High-Speed Atomic Force Microscopy (HS-AFM) Videography. Journal of extracellular vesicles. 2025. 14. 4. e270050
Goro Nishide, Keesiang Lim, Akiko Kobayashi, Yujia Qiu, Masaharu Hazawa, Toshio Ando, Yuki Okada, Richard W Wong. Spatiotemporal dynamics of protamine-DNA condensation revealed by high-speed atomic force microscopy. Nucleic Acids Research. 2025. 53. 6. gkaf152-1-gkaf152-11
Mingbo Qu, Lin Kong, Pengfei Li, Aijing Zhang, Toshio Ando, Qing Yang. High-speed atomic force microscopy reveals opposite traffic of processive chitinases impairs α-chitin biodegradation. Carbohydrate Polymers. 2025. 352. 123176-1-123176-10
Daiki Muramatsu, Takahiro Watanabe-Nakayama, Mayumi Tsuji, Kenichi Umeda, Sadao Hikishima, Hiroto Nakano, Yasuhiro Sakashita, Tokuhei Ikeda, Hiroki Konno, Noriyuki Kodera, et al. ALZ-801 prevents amyloid β-protein assembly and reduces cytotoxicity: A preclinical experimental study. The FASEB Journal. 2025. 39. e70382-01-e70382-13
Mingbo Qu, Xiaoxi Guo, Toshio Ando, Qing Yang. Functional role of carbohydrate-binding modules in multi-modular chitinase OfChtII. Journal of Biological Chemistry. 2024. 300. 9. 107622-107622

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MISC (103)：

小田隆, 大井里香, 古寺哲幸, 安藤敏夫, 小沼剛, 菅瀬謙治, 苙口友隆, 井上倫太郎, 杉山正明, 石野園子, et al. Integrated approach using small angle X-ray scattering and computational analysis for understanding dynamic structure and function of intrinsically disordered protein. 日本農芸化学会大会講演要旨集(Web). 2022. 2022
ANDO Toshio, ITO Yutaka, SUGITA Yuji, NANGO Eriko, YASUNAGA Takuo, OKADA Yasushi, KANDA Genki N. New Technologies that Propel Biophysics. Seibutsu Butsuri. 2021. 61. 2. 111-118
西出梧朗, 西出梧朗, LIM Keesiang, MOHAMED Mahmoud Shaaban, MOHAMED Mahmoud Shaaban, 小林亜紀子, 小林亜紀子, 羽澤勝治, 羽澤勝治, 中山隆宏, et al. Real-time Imaging of Histone H2A Wrapping Dynamics with DNA Inchworming using High-Speed Atomic Force Microscopy. 日本細胞生物学会大会(Web). 2021. 73rd
西出梧朗, LIM Keesiang, 小林亜紀子, 小林亜紀子, 羽澤勝治, 羽澤勝治, 中山隆宏, 古寺哲幸, 安藤敏夫, WONG Richard, et al. 高速AFMを用いたヒストンH2A-DNA実時空間イメージングによるヌクレオソーム凝縮プロセスとその環境の解明. 日本生化学会大会(Web). 2021. 94th
紺野宏記, 羽澤勝治, 芳坂綾子, 佐々木理乃, 矢幡信成, 古寺哲幸, 安藤敏夫. Investigation of formation process of Prx high molecular weight complexes and its physiological significance. 日本生体エネルギー研究会討論会講演要旨集. 2020. 46th

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Patents (19)：

液体充填方法、SICM用プローブの製造方法、SICM用プローブ及び SICM
Abtastvorrichtung für Rastersondenmikroskop
原子間力顕微鏡、制御方法、及び、プログラミング
プローブ走査機構、プローブ装置および走査プローブ顕微鏡
電気生理学的解析を可能にした原子間力顕微鏡

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Books (23)：

High-speed ion conductance microscopy (Page 521-534, Chapter in Encyclopedia of Solid-Liquid Interfaces. Vol.1)
Elsevier 2023 ISBN:9780323856690
High-speed atomic force microscopy in biology
Springer Berlin, Heidelberg 2022 ISBN:9783662647837
Myosins: A superfamility of Molecular Motors (ed. Lynne Coluccio)
Springer 2020
実験医学増刊 Vol38-No.5 イメージング時代の構造生命科学(田中啓二、若槻荘市編集)
羊土社 2020
Direct imaging of walking myosin V by high-speed atomic force microscopy
Springer Science+Business Media, LLC, part of Springer Nature 2018

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Lectures and oral presentations (148)：

High-speed AFM for dynamic structural biology
(EMBL Workshop: In-situ structural biology: expanding the toolbox for structural cell biology (Heidelberg, Germany) 2025)
Energy transduction mechanism in walking myosin V studied with interactive high-speed AFM
(Linz Winter Workshop 2025 (Linz Austria) 2025)
高速AFMの基礎からバイオ応用まで
(応用物理学会第53回薄膜・表面物理基礎講座 2024)
Plenary IUPAB Engstrom Lecture "Filming biomolecules in dynamic action by high-speed AFM"
(IUPAB 2024 Kyoto (21st IUPAB & 62nd BSJ Joint Congress) (Kyoto, Japan) 2024)
高速AFMの技術と応用に関する最近の動向
(【第4回】高速AFMシンポジウム高速AFMによる生命活動観察 ~最新研究とその観察手法について~ 2024)

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Education (2)：

1975 - 1980 Waseda University Graduate School of Science and Engineering Physics
1970 - 1974 Waseda University School of Science and Engineering Applied Physics

Professional career (2)：

Doctor of Science (Waseda University)
名誉博士 (AIX-Marseille University)

Work history (8)：

2022/03 - 現在 Kanazawa University
2017/10 - 現在 Kanazawa University Nano Life Science Institute (WPI-NanoLSI) Specially-appointed Professor
1996/07 - 2016/03 Kanazawa University Department of Physics Professor
2010/10 - 2015/03 Kanazawa University Bio-AFM Frontier Research Center Director
1992 - 1996 Kanazawa University Department of Physics Associate Professor

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Awards (22)：

2024/11 - Leo Easki Prize
2023/04 - Japan Medal with Purple Ribbon Development of High-speed Atomic Force Microscopy
2023/03 - Toray Science Foundation Toray Science and Technology Prize
2022/03 - 内藤記念科学振興財団内藤記念科学振興賞高速原子間力顕微鏡の開発と蛋白質の機能メカニズムの解明
2021/05 - 石川テレビ放送株式会社石川テレビ賞高速原子間力顕微鏡の開発とタンパク質の機能メカニズムの解明

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Association Membership(s) (2)：

アメリカ生物物理学会 , THE BIOPHYSICAL SOCIETY OF JAPAN

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