Rchr
J-GLOBAL ID:201201098932605000
Update date: Sep. 28, 2024
Shiroishi Mitsunori
シロイシ ミツノリ | Shiroishi Mitsunori
Affiliation and department:
Job title:
Associate Professor
Homepage URL (1):
https://shiroishimitsunori.wixsite.com/shiroishilab-jp
Research field (4):
Biophysics
, Applied biofunctional and bioprocess engineering
, Functional biochemistry
, Structural biochemistry
Research keywords (7):
receptor, antibody, immunology, G-protein coupled receptor, membrane protein
, protein engineering, antibody engineering
, protein engineering
, immunoreceptor
, 構造解析
, 抗体
, GPCR
Research theme for competitive and other funds (13):
- 2019 - 2022 Elucidation of the molecular recognition of stereotype rheumatoid factor.
- 2015 - 2018 Creation of the membrane-protein-particle antigen for the effective production of a crystallization-facilitator antibody
- 2014 - 2018 Establishment of research foundation on "objective pain assessment" using animal models suffering from neuropathic pain
- 2015 - 2017 Fundamental Study on Preparations for Therapeutic Antibodies with Less Productions of Anti-Drug Antibodies
- 2013 - 2017 Development of the strategy for creating stabilized GPCR variants for large-scale preparation
- 2013 - 2016 Crystallization of Angiotensin receptor type 2 with its specific antibody
- 2011 - 2014 Study on the development of molecules for depressing neuropathic pain based on tertiary structure of human P2X4 receptor
- 2011 - 2012 Elucidating of inhibition mechanism of anti-histamines for the histamine receptor by X-ray crystallography.
- 2011 - 2012 Development of Fab molecule with the stable scaffold for next generation
- 2010 - 2012 Crystallization of GPCR and its specific antibody
- 2009 - 2011 Elucidation of the transport mechanism of a secondary active transporter, Mhp1.
- 2005 - 2006 蛋白質間相互作用と立体構造解析に基づく免疫細胞受容体LIR/ILT群の機能解明
- 2000 - 2002 構造情報を基盤とした蛋白性抗原と抗体間相互作用の精密解析
Show all
Papers (69):
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Kaneko H, Korenaga R, Nakamura R, Kawai S, Ando T, Shiroishi M. Binding characteristics of the doxepin E/Z-isomers to the histamine H1 receptor revealed by receptor-bound ligand analysis and molecular dynamics study. JOURNAL OF MOLECULAR RECOGNITION. 2024. e3098
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Takasawa S, Kimura K, Miyanaga M, Uemura T, Hachisu M, Miyagawa S, Ramadan A, Sukegawa S, Kobayashi M, Kimura S, Matsui K, Shiroishi M, Terashita K, Nishiyama C, Yashiro T, Nagata K, Higami Y, Arimura GI. The powerful potential of amino acid menthyl esters for anti-inflammatory and anti-obesity therapies. IMMUNOLOGY. 2024. 13798
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Maruto Yamamoto, Ryoma Funada, Ryota Taki, Mitsunori Shiroishi. Production and characterization of an Fv-clasp of rheumatoid factor, a low-affinity human autoantibody. JOURNAL OF BIOCHEMISTRY. 2023
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Ayami Watanabe, Ami Nakajima, Mitsunori Shiroishi. Recovery of the histamine H3 receptor activity lost in yeast cells through error-prone PCR and in vivo selection. Scientific Reports. 2023. 13
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Nakagawa F, Kikkawa M, Chen S, Miyashita Y, Hamaguchi-Suzuki N, Shibuya M, Yamashita S, Nagase L, Yasuda S, Shiroishi M, Senda T, Ito K, Murata T, Ogasawara S. Anti-nanodisc antibodies specifically capture nanodiscs and facilitate molecular interaction kinetics studies for membrane protein. Scientific Reports. 2023. 13
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MISC (42):
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Ryota Nakamura, Shinnosuke Kawai, Hiroto Kaneko, Ryunosuke Korenaga, Tadashi Ando, Mitsunori Shiroishi. Free energy calculations of E- and Z-doxepin isomers binding to histamine H1 receptor. SEIBUTSU BUTSURI. 2023. 63. SUPPLEMENT1-2. S309-S309
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Hiroto Kaneko, Ryunosuke Korenaga, Ryota Nakamura, Shinnosuke Kawai, Tadashi Ando, Mitsunori Shiroishi. Binding properties of E/Z-Doxepin isomers to histamine H1 receptor. SEIBUTSU BUTSURI. 2023. 63. SUPPLEMENT1-2. S309-S309
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白石充典, 松原直紀, 安田賢司, 齊藤秀俊, 津田誠, 木下正弘, 村田武士, 植田正. Stabilization and large-scale preparation of P2Y12 receptor using theoretical prediction method and Saccharomyces cerevisiae expression system. 日本分子生物学会年会プログラム・要旨集(Web). 2021. 44th
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扇谷和希, 佐藤愼吾, 鈴木七緒, 安田賢司, 白石充典, 木下正弘, 村田武士. メラトニン1A受容体の高発現・熱安定化改変体の創出. 日本生体エネルギー研究会討論会講演要旨集. 2018. 44th
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中野僚介, 肥喜里志門, 肥喜里志門, 安田賢司, 安田賢司, 白石充典, 木下正弘, 村田武士. GPCR活性型変異体スクリーニング系の構築. 日本生体エネルギー研究会討論会講演要旨集. 2018. 44th
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Books (10):
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2022年度版 基礎工学実験
学術図書出版 2022 ISBN:9784780610062
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膜タンパク質工学ハンドブック
株式会社エヌ・ティ・エス 2020 ISBN:9784860435370
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Esentialタンパク質科学
南江堂 2016
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津本浩平、植田正、前仲勝実監訳「Esentialタンパク質科学」
南江堂 2016
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進化を続ける構造生物学
化学同人 2014
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Lectures and oral presentations (110):
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出芽酵母を利用した膜タンパク質工学
(21世紀を明るく科学する会(2024) 2024)
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リウマトイド因子RF-TS1におけるVL-VH間の不安定性と分子認識の関係
(日本薬学会第144年会 2024)
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ヒスタミンH1受容体に対するE/Z-Doxepin立体異性体の結合評価
(日本薬学会第144年会 2024)
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抗D-アスパラギン酸抗体のFv-claspの調製と複合体の結晶化
(第46回日本分子生物学会年会 2023)
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出芽酵母において喪失したヒスタミンH3受容体のシグナル活性を回復させる変異の評価
(第46回日本分子生物学会年会 2023)
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Education (3):
- 2000 - 2003 Tohoku University Graduate School of Engineering
- 1998 - 2000 Tohoku University Graduate School of Engineering
- 1994 - 1998 Tohoku University
Professional career (1):
Work history (6):
Committee career (1):
- 2022/04/01 - 2026/03/31 PF-UAタンパク質結晶構造解析ユーザーグループ 幹事
Awards (5):
- 2017/03 - 第28回加藤記念研究助成 優秀賞
- 2013/05 - 日本生化学会九州支部会 学術奨励賞
- 2012/04 - 文部科学大臣表彰若手科学者賞
- 2001/08 - Poster award
- 2001/08 - Finn Wold travel award
Association Membership(s) (3):
THE PHARMACEUTICAL SOCIETY OF JAPAN
, PROTEIN SCIENCE SOCIETY OF JAPAN
, THE JAPANESE BIOCHEMICAL SOCIETY
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