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J-GLOBAL ID：201501010204744434 Update date: Feb. 23, 2025

Takashi Fujishiro

フジシロタカシ | Takashi Fujishiro

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Affiliation and department：
Homepage URL (1)： https://kaken.nii.ac.jp/d/r/20740450.ja.html

Research field (9)： Applied biochemistry , Functional biochemistry , Structural biochemistry , Bioorganic chemistry , Applied microbiology , Inorganic and coordination chemistry , Nanobioscience , Chemical biology , Molecular biochemistry

Research keywords (16)： Amino acid metabolism , 金属ポルフィリン , 金属クラスター , CO dehydrogenase (CODH) , Hybrid cluster protein (HCP) , coenzyme F430 , PLP酵素 , Chelatase , metal cofactor , Nickel , Biosynthesis , Enzyme catalysis , X-ray crystal structure analysis , Hydrogenase , Iron-sulfur cluster , Metalloprotein

Research theme for competitive and other funds (8)：

2023 - 2025 三重結合アミノ酸生合成系を改変したクリックケミストリー指向型ペプチド合成系の構築
2023 - 2025 COデヒドロゲナーゼ(CODH)の祖先型酵素HCPがCO代謝活性を得る分子進化の変異の再現
2022 - 2025 Characterization of the unique Fe-S-O metallocluster of HCP enzyme
2020 - 2024 鉄硫黄クラスター生合成マシナリーの反応過程における過渡的な中間状態の解明
2019 - 2021 Unraveling the catalytic mechanisms of Ni-prosthetic-group F430 biosynthetic enzymes

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Papers (42)：

Ryosuke Nakamura, Takashi Fujishiro. Visualizing thiazolidine ring formation in the reaction of D-cysteine and pyridoxal-5′-phosphate within L-cysteine desulfurase SufS. Biochemical and Biophysical Research Communications. 2025. 151497-151497
Shoko Ogawa, Masahide Hikita, Takashi Fujishiro. Structural insights into the recognition of tetrapyrrole substrates by ancestral class II chelatase CfbA. Protein Science. 2024. 33. 12. e5208
Takuya Terahata, Yukino Shimada, Chisato Maki, Suguru Muroga, Rina Sakurai, Kouhei Kunichika, Takashi Fujishiro. Cysteine-Persulfide Sulfane Sulfur-Ligated Zn Complex of Sulfur-Carrying SufU in the SufCDSUB System for Fe-S Cluster Biosynthesis. Inorganic Chemistry. 2024. 63. 42. 19607-19618
Yuuma Oyamada, Shoko Ogawa, Takashi Fujishiro. Nickel-chelatase activity of SirB variants mimicking the His arrangement in the naturally occurring nickel-chelatase CfbA. FEBS Open Bio. 2024. 14. 8. 1291-1302
Takaomi Sasaki, Saya Takita, Takashi Fujishiro, Yunosuke Shintani, Satoki Nojiri, Ryota Yasui, Tetsuro Yonesaki, Yuichi Otsuka. Phage single-stranded DNA-binding protein or host DNA damage triggers the activation of the AbpAB phage defense system. mSphere. 2023. 8. 6. e0037223

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MISC (12)：

Maya Murata, Taichi Murakami, Eiki Yuda, Nanami Mukai, Xintong Zheng, Natsumi Kurachi, Sachiko Mori, Shoko Ogawa, Kouhei Kunichika, Takashi Fujishiro, et al. The minimal SUF system can substitute for the canonical iron-sulfur cluster biosynthesis systems by using inorganic sulfide as the sulfur source. 2024
FUJISHIRO Takashi, NAKAMURA Ryosuke, TAKAHASHI Yasuhiro. Sulfur-mobilizing Enzymes Involved in Iron-sulfur Cluster Biosynthesis: Shared Structural Features and Functional Diversity. Seibutsu Butsuri. 2021. 61. 3. 180-182
藤城貴史. 埼玉新聞サイ・テクこらむ-金属と生命、そして進化. 埼玉新聞. 2019
藤城貴史. Chem-Station第133回スポットライトリサーチ「システインから無機硫黄を取り出す酵素反応の瞬間を捉える」. Chem-Station. 2018
Bioinorganic chemistry on biosynthesis of metal clusters in metalloproteins. 2018. 9. 17-20

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Books (3)：

Bioorganometallic Chemistry
De Gruyter 2020
Biohydrogen, (Edited by Prof. Dr. Matthias Rögner)
De Gruyter 2015
Construction of monooxygenation systems using hydrogen peroxide-dependent cytochrome P450s
2011

Lectures and oral presentations (89)：

金属-テトラピロール補因子の生合成酵素キラターゼの構造と機能の進化の追跡
(第97回日本生化学会大会 2024)
Challenges for molecular design of Fe-S enzymes functioning as an artificial Ni,Fe-CO dehydrogenase
(The 2nd CO world Plenary Meeting 2024)
三重結合アミノ酸生合成系を改変したクリックケミストリー指向型ペプチド合成系の構築
(学術変革領域A「予知生合成科学」第4回公開シンポジウム 2024)
PLP依存型システイン脱硫酵素IscSの活性の起源の解明
(第50回生体分子科学討論会 2024)
Study on E. coli recombinant metalloproteins with use of synthetic genes
(2024 annual meeting of Japan Scociety for Bioscience, Biotechnology, and Agrochemistry 2024)

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Education (2)：

2006 - 2011 Nagoya University Graduate School of Science Department of Chemistry
2002 - 2006 Nagoya University School of Science Department of Chemistry

Professional career (1)：

博士(理学) (名古屋大学)

Work history (4)：

2024/04 - 現在 Saitama University Graduate School of Science and Engineering Professor
2020/04 - 2024/03 Saitama University Graduate School of Science and Engineering Associate Professor
2015/04 - 2020/03 Saitama University Graduate School of Science and Engineering Assistant Professor
2011/04 - 2015/03 Max Planck Institute for Terrestrial Microbiology Postdoc

Awards (6)：

2014/08 - Young researcher oral presentation prize, at the EuroBIC12
2012/03 - The oral prsentation award for young scientists in the 92nd annual meeting of Chemical Society of Japan
2010/11 - AsBIC V, Student Travel Award
2009/08 - A poster award for students in the summer school of the Japan Society of Coordination Chemistry
2009/03 - The oral prsentation award for students in the 89th annual meeting of Chemical Society of Japan

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Association Membership(s) (5)：

JAPAN SOCIETY OF COORDINATION CHEMISTRY , PROTEIN SCIENCE SOCIETY OF JAPAN , JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY , THE JAPANESE BIOCHEMICAL SOCIETY , THE CHEMICAL SOCIETY OF JAPAN

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