2017 - 2020 Development of a DNA methylation-based body fluid-specific genotyping method
2014 - 2017 Development of methods for body fluid identification based on DNA methylation.
2012 - 2014 Demelopment of a realtime PCR-based semen identification method using the semen-specific hypomethylated DNA region
Papers (46):
Kochi TOYOMANE, Tomoko AKUTSU, Ken WATANABE, Takayuki YAMAGISHI, Satoshi KUBOTA. Potential application of Staphylococcus species detection in the specific identification of saliva. Legal Medicine. 2023. 102320-102320
Tomoko Akutsu, Isao Yokota, Ken Watanabe, Kochi Toyomane, Takayuki Yamagishi, Koichi Sakurada. 単一の体液の同定を目的としたSingle-target multiplex RT-PCR法の検証-5種の体液を対象としたMulti-target multiplex RT-PCR法との比較-. Japanese Journal of Forensic Science and Technology. 2023
Takayuki Yamagishi, Yuya Hamano, Ken Watanabe, Chie Morimoto, Kochi Toyomane, Tomoko Akutsu. DNAメチル化率を指標としたBekaertおよびGaraliの年齢推定法の日本人血液および陳旧血痕試料における検証. Japanese Journal of Forensic Science and Technology. 2023
Kei Taniguchi, Tomoko Akutsu, Ken Watanabe, Yoshinori Ogawa, Kazuhiko Imaizumi. A vertebrate-specific qPCR assay as an endogenous internal control for robust species identification. Forensic science international. Genetics. 2022. 56. 102628-102628
Ken Watanabe, Kochi Toyomane, Takayuki Yamagishi, Tomoko Akutsu. Developmental validation of an ABO genotyping method using TaqMan multiplex real-time PCR. Japanese Journal of Forensic Science and Technology. 2022
Verification of anti-Tamm-Horsfall protein (uromucoid) antibodies applicable to identify urine by indirect enzyme-linked immunosorbent assay. 2014. 63. 1. 9-13
Identification of Body Fluid Stains Using Real-time RT-PCR : Discrimination between Salivary, Nasal, and Vaginal Secretions. 2013. 18. 1. 1-11