Art

J-GLOBAL ID：200902173815223571   Reference number：02A0767087

Extremely Simple, Rapid, and Highly Efficient Transformation Method for the Yeast Saccharomyces cerevisiae Using Glutathione and Early Log Phase Cells.

グルタチオンと対数増殖期初期細胞を用いたきわめて簡便で迅速で非常に効率的なSaccharomyces cerevisiaeの形質転換方法

Author (5)： HAYAMA Y (Kyoto Univ., Kyoto, JPN) ,  FUKUDA Y (Chukyo Community Coll., Gifu, JPN) ,  KAWAI S (Kyoto Univ., Kyoto, JPN) ,  HASHIMOTO W (Kyoto Univ., Kyoto, JPN) ,  MURATA K (Kyoto Univ., Kyoto, JPN)
Material： Journal of Bioscience and Bioengineering  (J Biosci Bioeng)
Volume： 94  Issue： 2  Page： 166-171  Publication year： Aug. 25, 2002 
JST Material Number： G0535B  ISSN： 1389-1723  CODEN： JBBIF6  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： tripeptide ,...

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JST classification (1)： Gene manipulation  (EC02050B)

Substance index (2)： Glutathione ,  Oxidized glutathione

Reference (14)：

• 1. Ito, H., Fukuda, Y., Murata, K., and Kimura, A.: Transformation of intact yeast cells treated with alkali cations. J. Bacteriol., 153, 163–168 (1983).
• 2. Hinnen, A., Hicks, J. B., and Fink, G. R.: Transformation of yeast. Proc. Natl. Acad. Sci. USA, 75, 1929–1933 (1978).
• 3. Becker, O. M. and Guarente, L.: High-efficiency transformation of yeast by electroporation. Methods Enzymol., 194, 182–187 (1991).
• 4. Tajima, M., Nogi, Y., and Fukusawa, T.: Primary structure of the Saccharomyces cerevisiae GAL7 gene. Yeast, 1, 267–277 (1985).
• 5. Suizu, T., Tsutsumi, H., Ohtake, Y., Kawado, A., Imayasu, S., Kimura, A., and Murata, K.: Absolute glutathione requirement for sporulation of a yeast Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun., 205, 1151–1155 (1994).

Terms in the title (6)： グルタチオン ,  対数増殖期 ,  初期 ,  細胞 ,  Saccharomyces ,  形質転換