Art

J-GLOBAL ID：201802237052000165   Reference number：18A0811201

Plant tissue clearing for fluorescence imaging

透明化技術を用いた植物組織蛍光観察のすすめ

Author (4)： KURIHARA DAISUKE (名古屋大学大学院理学研究科) ,  KURIHARA DAISUKE (JST, ERATO東山ライブホロニクスプロジェクト) ,  MIZUTA YOKO (名古屋大学トランスフォーマティブ生命分子研究所) ,  MIZUTA YOKO (JST, さきがけ)
Material： Plant Morphology  (Plant Morphol)
Volume： 29  Issue： 1  Page： 81-86(J-STAGE)  Publication year： 2017 
JST Material Number： L7959A  ISSN： 0918-9726  Document type： Article
Article type： 解説  Country of issue： Japan (JPN)  Language： JAPANESE (JA)

Thesaurus term： property ,...
Semi thesaurus term： *fluorescence microscopy ,...

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Author keywords (10)： clearing ,  confocal microscopy ,  deep imaging ,  fluorescent protein ,  two-photon microscopy ,  clearing ,  confocal microscopy ,  deep imaging ,  fluorescent protein ,  two-photon microscopy

JST classification (2)： Research methods in botany  (EH01020C) ,  Microscopic methods of living organisms  (EA03050C)

Reference (16)：

• Aoyagi, Y., Kawakami, R., Osanai, H., Hibi, T., and Nemoto, T. (2015) A rapid optical clearing protocol using 2,2’-thiodiethanol for microscopic observation of fixed mouse brain. PLoS One 10: e0116280.
• Hama, H., Kurokawa, H., Kawano, H., Ando, R., Shimogori, T., Noda, H., Fukami, K., Sakaue-Sawano, A., and Miyawaki, A. (2011) Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain. Nat Neurosci 14: 1481-1488.
• Hasegawa, J., Sakamoto, Y., Nakagami, S., Aida, M., Sawa, S., and Matsunaga, S. (2016) Three-dimensional imaging of plant organs using a simple and rapid transparency technique. Plant Cell Physiol 57: 462-472.
• Hoyer, H. (1882) Beiträge zur histologischen Technik. Biol Centralbl 2: 23-24.
• Kumar, R. and Silva, L. (1973) Light ray tracing through a leaf cross section. Appl Optics 12: 2950-2954.

Terms in the title (2)： 植物 ,  観察