Art

J-GLOBAL ID：202002226799032798   Reference number：20A1312369

A stable protocol for the fabrication of transplantable human oral mucosal epithelial cell sheets for clinical application

臨床応用のための移植可能なヒト口腔粘膜上皮細胞シート作製のための安定したプロトコル

Author (21)： Kasai Yoshiyuki (Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-Cho, Shinjuku-ku, Tokyo, 162-8666, Japan) ,  Kasai Yoshiyuki (Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University (TWIns), 2-2 Wakamatsu-Cho, Shinjuku-ku, Tokyo, 162-8480, Japan) ,  Takagi Ryo (Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-Cho, Shinjuku-ku, Tokyo, 162-8666, Japan) ,  Kobayashi Shinichiro (Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-Cho, Shinjuku-ku, Tokyo, 162-8666, Japan) ,  Kobayashi Shinichiro (Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki-Shi, Nagasaki, 852-8501, Japan) ,  Owaki Toshiyuki (Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-Cho, Shinjuku-ku, Tokyo, 162-8666, Japan) ,  Yamaguchi Naoyuki (Department of Gastroenterology and Hepatology, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki-Shi, Nagasaki, 852-8501, Japan) ,  Fukuda Hiroko (Department of Gastroenterology and Hepatology, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki-Shi, Nagasaki, 852-8501, Japan) ,  Sakai Yusuke (Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki-Shi, Nagasaki, 852-8501, Japan) ,  Sumita Yoshinori (Department of Regenerative Oral Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki-Shi, Nagasaki, 852-8501, Japan) ,  Kanai Nobuo (Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-Cho, Shinjuku-ku, Tokyo, 162-8666, Japan) ,  Isomoto Hajime (Department of Gastroenterology and Hepatology, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki-Shi, Nagasaki, 852-8501, Japan) ,  Kanetaka Kengo (Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki-Shi, Nagasaki, 852-8501, Japan) ,  Ohki Takeshi (Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-Cho, Shinjuku-ku, Tokyo, 162-8666, Japan) ,  Asahina Izumi (Department of Regenerative Oral Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki-Shi, Nagasaki, 852-8501, Japan) ,  Nagai Kazuhiro (Transfusion and Cell Therapy Unit, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki-Shi, Nagasaki, 852-8501, Japan) ,  Nakao Kazuhiko (Department of Gastroenterology and Hepatology, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki-Shi, Nagasaki, 852-8501, Japan) ,  Takeda Naoya (Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University (TWIns), 2-2 Wakamatsu-Cho, Shinjuku-ku, Tokyo, 162-8480, Japan) ,  Okano Teruo (Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-Cho, Shinjuku-ku, Tokyo, 162-8666, Japan) ,  Eguchi Susumu (Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki-Shi, Nagasaki, 852-8501, Japan) ,  Yamato Masayuki (Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University (TWIns), 8-1 Kawada-Cho, Shinjuku-ku, Tokyo, 162-8666, Japan)
Material： Regenerative Therapy (Web)
Volume： 14  Page： 87-94  Publication year： 2020 
JST Material Number： U0955A  ISSN： 2352-3204  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Thesaurus term： human ,...
Semi thesaurus term： cell harvest

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Author keywords (4)： Primary cell culture ,  Minimum trypsinization ,  Epithelial cell sheet ,  Clinical application

JST classification (2)： Medical materials  (GA05040H) ,  Cell and tissue culture methods  (EA03040R)

Terms in the title (8)： 臨床 ,  応用 ,  移植 ,  ヒト ,  口腔 ,  粘膜上皮細胞 ,  作製 ,  プロトコル