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J-GLOBAL ID：202002256043963135   Reference number：20A2486307

Establishment of human immortalized mesenchymal stem cells lines for the monitoring and analysis of osteogenic differentiation in living cells

生細胞における骨形成分化のモニタリングと分析のためのヒト不死化間葉系幹細胞株の樹立【JST・京大機械翻訳】

Author (7)： Narai Takashi (Division of Oral and Maxillofacial Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine, Yonago, 683-8504, Japan) ,  Watase Ryohei (Division of Human Genome Science, Department of Molecular and Cellular Biology, School of Life Sciences, Tottori University Faculty of Medicine, Yonago, 683-8503, Japan) ,  Nakayama Yuji (Division of Radioisotope Science, Research Initiative Center, Organization for Research Initiative and Promotion, Tottori University, Yonago, 683-8503, Japan) ,  Kodani Isamu (Division of Oral and Maxillofacial Surgery, Department of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine, Yonago, 683-8504, Japan) ,  Inoue Toshiaki (Division of Human Genome Science, Department of Molecular and Cellular Biology, School of Life Sciences, Tottori University Faculty of Medicine, Yonago, 683-8503, Japan) ,  Kokura Kenji (Division of Human Genome Science, Department of Molecular and Cellular Biology, School of Life Sciences, Tottori University Faculty of Medicine, Yonago, 683-8503, Japan) ,  Kokura Kenji (Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba, 274-8510, Japan)
Material： Heliyon
Volume： 6  Issue： 10  Page： Null  Publication year： 2020 
JST Material Number： W3098A  ISSN： 2405-8440  Document type： Article
Article type： 原著論文  Country of issue： Netherlands (NLD)  Language： ENGLISH (EN)

Abstract/Point： Mesenchymal stem cells (MSCs) ...

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Thesaurus term： *osteogenesis ,  calcification ,  human ,  *differentiation (biology) ,  dyeing ,  Up-Regulation ,  Cell Differentiation ,  in vitro experiment ,  osteoblast ,  *mesenchymal cell ,  Osteocalcin ,  endogenous ,  *live cell ,  protein expression ,  enhanced Green Fluorescent Protein ,  developmental physiology
Semi thesaurus term： EGFP , 【Automatic Indexing@JST】

Author keywords (11)： Cell culture ,  Cell differentiation ,  Stem cell research ,  Cell biology ,  Promoter ,  Regenerative medicine ,  Bone gamma-carboxyglutamate protein ,  CRISPR-Cas9 ,  Mesenchymal stem cells ,  Osteogenic differentiation ,  Reporter cells

JST classification (2)： Cell physiology in general  (EF02010S) ,  Development(=generation)and differentiation  (EJ16020W)

Terms in the title (8)： 生細胞 ,  骨形成分化 ,  モニタリング ,  分析 ,  ヒト ,  不死化 ,  間葉系幹細胞 ,  株