Tokunaga Makio

トクナガマキオ | Tokunaga Makio

Affiliation and department：
Job title： Professor

Research field (1)： Molecular biology

Research keywords (3)： 1分子計測・操作 , Single-molecule measurements and manipulation , Bioimaging

Research theme for competitive and other funds (29)：

2022 - 2025 Development of integrative methods for correlative studies among dynamics, nano-localization, and function using simultaneous multi-color single-molecule and super-resolution imaging
2019 - 2022 Elucidation of the regulatory mechanism of phase separation for transcliption by developing a correlative analysis method of nano-scale localization and dynamics
2015 - 2018 International research network for chromatin structure, dynamics, and function
2013 - 2018 Nanoanalysis of the molecular mechanism of chromatin functions using single molecule imaging in vio and super-resolution microscopy
2013 - 2018 Chromatin structure, dynamics, and function

2012 - 2017 Elucidation of mechanisms of transcription cycle using multi-color, single-molecule, quantitative fluorescence imaging of living cell nuclei.

2013 - 2016 Nanoquantification of the regulatory mechanism of molecular dynamics by development of super-resolution microscopy using single molecule imaging

2008 - 2014 The physicochemical field for genetic activities

2008 - 2012 In-situ real-time measurement of biological informations using single molecule imaging

2009 - 2011 Innovative research on molecular systems of immune cells using combination of single molecule imaging and numerical modeling

2008 - 2009 局所的翻訳調節によるシナプス形成・可塑性制御メカニズムの解析

2007 - 2008 Single molecule imaging and quantitative analysis of initial activation process in immune cells

2006 - 2007 Quantification of molecular dynamics, numbers and interactions of multi-kind molecules by single molecule imaging in cells

2006 - 2007 1分子イメージングによる細胞内輸送の動態解析

2006 - 2007 シナプス可塑性における局所的翻訳を制御する分子メカニズムの解析

2005 - 2006 Single molecule imaging analysis on antigen recognition and activation of T lymphocytes.

2005 - 2005 シナプス可塑性における局所的翻訳制御機構の解析

2003 - 2004 神経樹状突起mRNA輸送複合体を構成するp105新規タンパク質の機能解析

1999 - 2003 分子間相互作用の可視化と細胞内分子定量イメージング

2002 - 2002 p105新規タンパク質による細胞質mRNA複合体の形成と細胞骨格依存的輸送

1999 - 1999 アクチン線維束に介したHIV-1 RevによるウイルスmRNAの核外輸送

1999 - 1999 分子モーター間に働く相互作用の1分子イメージング

1998 - 1999 Single molecule research of molecular interactions using scanning probe microscopy

1998 - 1998 分子モーター間に働く相互作用の1分子イメージング

1992 - 1992 アクチン・ミオシン系の動的相互作用の分子機構

1991 - 1992 レ-ザ-顕微検査器の開発と薬物・情報伝達物質による細胞の応答・損傷の解析

1990 - 1990 蛍光プローブを用いた情報伝達機構における分子間相互作用の研究

1990 - 1990 蛋白質・情報伝達物質の高感度運動測定による細胞内情報伝達処理機構の研究

1989 - 1989 ミオシン頭部の立体構造変化の研究

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Papers (124)：

Yuma Ito, Masanori Hirose, Makio Tokunaga. Slitflow: a Python framework for single-molecule dynamics and localization analysis. 2023
Haruka Matsumori, Kenji Watanabe, Hiroaki Tachiwana, Tomoko Fujita, Yuma Ito, Makio Tokunaga, Kumiko Sakata-Sogawa, Hiroko Osakada, Tokuko Haraguchi, Akinori Awazu, et al. Ribosomal protein L5 facilitates rDNA-bundled condensate and nucleolar assembly. Life science alliance. 2022. 5. 7
Satoshi Uchino, Yuma Ito, Yuko Sato, Tetsuya Handa, Yasuyuki Ohkawa, Makio Tokunaga, Hiroshi Kimura. Live imaging of transcription sites using an elongating RNA polymerase II-specific probe. The Journal of cell biology. 2022. 221. 2
Yang Liu, Ning Zhao, Masato T. Kanemaki, Yotaro Yamamoto, Yoshifusa Sadamura, Yuma Ito, Makio Tokunaga, Timothy J. Stasevich, Hiroshi Kimura. Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells. Genes to Cells. 2021
Satoshi Uchino, Yuma Ito, Yuko Sato, Tetsuya Handa, Yasuyuki Ohkawa, Makio Tokunaga, Hiroshi Kimura. Visualizing transcription sites in living cells using a genetically encoded probe specific for the elongating form of RNA polymerase II. 2021

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MISC (72)：

伊藤由馬, 徳永万喜洋. 生細胞内の1分子動態と相互作用の定量解析. パリティ “特集:物理科学,この1年”. 2019. 34. 1. 72-74
淺川東彦, YANG Hui-Ju, 山本孝治, 大槻千鶴, 近重裕次, 近重裕次, 十川久美子, 十川久美子, 徳永万喜洋, 徳永万喜洋, et al. 分裂酵母における核膜孔複合体の構成と機能. 日本細胞生物学会大会要旨集. 2014. 66th
十川久美子, 徳永万喜洋. 免疫細胞表面の1分子イメージング計測. 現代化学. 2011. 488. 11. 42-43
Nobuyuki Shiina, Makio Tokunaga. Dendritic mRNA transport and local translation are responsible for the formation of neuronal networks. NEUROSCIENCE RESEARCH. 2011. 71. E74-E74
TOKUNAGA Makio, SAKATA SOGAWA Kumiko. Clear Single Molecular Imaging in Living Cells using Highly Inclined Thin Illumination. Seibutsu Butsuri. 2009. 49. 6. 318-321

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Patents (10)：

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Books (10)：

全反射照明蛍光顕微鏡 - 対物レンズ型 / 生きてるものは全部観る! イメージングの選び方・使い方100+
羊土社 2018 ISBN:4758103755
全反射照明蛍光顕微鏡 / 第4版現代界面コロイド化学の基礎
丸善 2018 ISBN:4621302914
1分子操作
化学同人 2014 ISBN:475981518X
分子でできた究極の微小モーター
講談社ブルーバックス 2010
ハートウェル遺伝学-遺伝子、ゲノム、そして生命システムへ-
メディカルサイエンスインターナショナル 2010 ISBN:9784895926256

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Lectures and oral presentations (118)：

Single-molecule localization-integrated trajectory analysis for dynamic interaction of chromatin structure and transcription machinery
(67th Biophysical Society Annual Meeting 2023)
RNA Polymerase II dynamics analysis at different stages of the transcription cycle in living cells using single-molecule imaging
(2022)
Single-molecule analysis of state-specific histone mobility in chromatin subcompartments during cellular differentiation
(2022)
A simulation study to measure precisely three-dimensional coordinates of single molecule images using adaptive optics and machine learning
(2022)
An integrated analysis of functional chromatin-RNA polymerase II interaction using singlemolecule dynamics and localization
(2022)

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Professional career (1)：

理学博士 (東京大学)

Work history (10)：

2016/04 - 現在 Tokyo Institute of Technology Life Science and Technology Professor
2008/04 - 2016/03 Tokyo Institute of Technology Graduate School of Bioscience and Biotechnology Professor
2013/04 - 2015/03 RIKEN Unit Leader
2004/04 - 2013/03 RIKEN Unit for Molecular Systems Immunology Unit Leader
2000/04 - 2008/03 National Institute of Genetics Structural Biology Center Professor

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Awards (2)：

2011/04 - Education, Culture, Sports, Science and Technology Prizes for Science and Technology, Research Category, The Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology Elucidation of molecular dynamic systems by development of single molecule microscopy
2007/06 - RIKEN RCAI (Research Center for Allergy and Immunology) Outstanding Contribution of the Year 2006 For the pioneering work to visualize the molecular dynamics within living lymphocytes and their discovery on the immunological functions of Microclusters.

Association Membership(s) (1)：

THE BIOPHYSICAL SOCIETY OF JAPAN

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