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J-GLOBAL ID：201701009168010964
Update date: Feb. 21, 2025 Ryo Amano
アマノ リョウ | Ryo Amano
The University of Tokyo The Institute of Medical Science
About The University of Tokyo The Institute of Medical Science
Research field (6)：
Pharmaceuticals - chemistry and drug development
, Chemical biology
, Molecular biochemistry
, Structural biochemistry
, Biophysics
, Molecular biology
Research keywords (11)：
RNAアプタマー
, SELEX
, ウイルス様粒子(VLP)
, RNA工学
, 進化分子工学
, 核酸医薬
, 核酸-蛋白質相互作用
, 蛋白質-蛋白質相互作用
, 表面プラズモン共鳴(SPR)解析
, 等温滴定型カロリメトリ(ITC)解析
, 核磁気共鳴法(NMR)
Research theme for competitive and other funds (2)： - 2022 - 2024 高度マルチプレックスSELEX法による機能性分子の限定的創製戦略の構築
- 2020 - 2022 RNA aptamers targeting dengue virus
- Kaku Goto, Ryo Amano, Akiko Ichinose, Akiya Michishita, Michiaki Hamada, Yoshikazu Nakamura, Masaki Takahashi. Generation of RNA aptamers against chikungunya virus E2 envelope protein. Journal of virology. 2025. e0209524
- Ryo Amano, Masaki Takahashi, Kazumi Haga, Mizuki Yamamoto, Kaku Goto, Akiko Ichinose, Michiaki Hamada, Jin Gohda, Jun-ichiro Inoue, Yasushi Kawaguchi, et al. A chimeric RNA consisting of siRNA and aptamer for inhibiting dengue virus replication. NAR Molecular Medicine. 2024. 1. 4
- Kazuyuki Kumagai, Hiroki Okubo, Ryo Amano, Tomoko Kozu, Masanori Ochiai, Masataka Horiuchi, Taiichi Sakamoto. Selection of aptamers using β-1,3-glucan recognition protein tagged proteins and curdlan beads. Journal of biochemistry. 2023. 174. 5. 433-440
- Masaki Takahashi, Ryo Amano, Michiru Ozawa, Anna Martinez, Kazumasa Akita, Yoshikazu Nakamura. Nucleic acid ligands act as a PAM and agonist depending on the intrinsic ligand binding state of P2RY2. Proceedings of the National Academy of Sciences of the United States of America. 2021. 118. 18
- Ryo Amano, Masato Namekata, Masataka Horiuchi, Minami Saso, Takuya Yanagisawa, Yoichiro Tanaka, Farhana Ishrat Ghani, Masakuni Yamamoto, Taiichi Sakamoto. Specific inhibition of FGF5-induced cell proliferation by RNA aptamers. Scientific reports. 2021. 11. 1. 2976-2976
- Ryo Amano, Akiya Michishita, Ryota Nakano, Akiko Ichinose, Kazumi Haga, Meng Ling Moi, Michiaki Hamada, Yoshikazu Nakamura, Masaki Takahashi. An aptamer neutralizing dengue virus generated by VLP-SELEX and advanced in silico analyses. 2023
- Ryo Amano, Akiya Michishita, Ryota Nakano, Akiko Ichinose, Kazumi Haga, Meng Ling Moi, Michiaki Hamada, Yoshikazu Nakamura, Masaki Takahashi. Generation of an RNA aptamer neutralizing dengue viruses by SELEX method targeting virus-like particles and advanced in silico analyses. INSNAC2023 Progtam & Abstracts. 2023
- Ryo Amano, Akiya Michishita, Ryota Nakano, Akiko Ichinose, Michiaki Hamada, Yoshikazu Nakamura, Masaki Takahashi. Selection strategy to generate aptamers against dengue viruses by Multi- VLP SELEX and an advanced in silico analysis. 2022
- Ryo Amano, Akiko Ichinose, Michiaki Hamada, Yoshikazu Nakamura, Masaki Takahashi. Generation of RNA aptamers against dengue viruses by a multiplex SELEX employing virus-like particles (VLPs). 2021
- 笹生みなみ, 天野亮, 行方昌人, 堀内正隆, 柳澤拓也, 西本翔, 田中陽一郎, GHANI Farhana Ishrat, 山本昌邦, 坂本泰一. 線維芽細胞増殖因子5(FGF5)に対するAptamerの解析. 日本分子生物学会年会プログラム・要旨集(Web). 2020. 43rd
Books (2)：
- Kinetic and Thermodynamic Analyses of RNA-Protein Interactions.
Humana Press 2020 - ITC Measurement for High-Affinity Aptamers Binding to Their Target Proteins
Humana Press 2019
- 2014 - 2017 千葉工業大学 博士後期課程
- 2017/12 - 現在 東京大学 医科学研究所 特任研究員
- 2013/07 - 2014/07 第15回若手NMR研究会 世話人
- 2024/12 - Oxford University Press (OUP), NAR Molecular Medicine EDITOR'S CHOICE A chimeric RNA consisting of siRNA and aptamer for inhibiting dengue virus replication
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