Waki Toshiyuki

ワキトシユキ | Waki Toshiyuki

Affiliation and department：
Job title： Assistant Professor
Homepage URL (1)： http://www.che.tohoku.ac.jp/~seika/

Research field (2)： Molecular biology , Applied molecular and cellular biology

Research keywords (4)：カルコン合成酵素 , タンパク質間相互作用 , フラボノイド , 植物特化代謝

Research theme for competitive and other funds (4)：

2023 - 2028 Molecular dissection of plant specialized metabolism machineries
2022 - 2025 ダイズ細胞内のイソフラボン配糖体の分解代謝機構の解明
2021 - 2024 フラボノイド生合成の細胞内動態を探る
2020 - 2022 陸上植物に進化的に保存されたカルコン合成酵素の活性制御機構の解明

Papers (24)：

Seiji Takahashi, Miki Suenaga-Hiromori, Tomoki Ishii, Nadia Nur Shazana Binti Abu Talib Khan, Tomoyo Mikami, Tomohiro Takahashi, Chiho Minakawa, Fumihiro Yanbe, Toshiyuki Waki, Toru Nakayama, et al. Biosynthesis of unnatural polyisoprenes by engineered prenyltransferases on rubber particles. 2024
Kazuhiro Ohara, Yasuhiro Oshima, Hideaki Unno, Satoru Nagano, Masami Kusunoki, Seiji Takahashi, Toshiyuki Waki, Satoshi Yamashita, Toru Nakayama. Lowering pH optimum of activity of SshEstI, a slightly alkaliphilic archaeal esterase of the hormone-sensitive lipase family. Journal of bioscience and bioengineering. 2024
Toshiyuki Waki, Riki Imaizumi, Kaichi Uno, Yamato Doi, Misato Tsunashima, Sayumi Yamada, Ryo Mameda, Shun Nakata, Taro Yanai, Kohei Takeshita, et al. Structural insights into catalytic promiscuity of chalcone synthase from Glycine max (L.) Merr.: Coenzyme A-induced alteration of product specificity. Biochemical and Biophysical Research Communications. 2024. 150080-150080
Riki Imaizumi, Hiroaki Matsuura, Taro Yanai, Kohei Takeshita, Shuto Misawa, Haruhiko Yamaguchi, Naoki Sakai, Yukino Miyagi-Inoue, Miki Suenaga-Hiromori, Toshiyuki Waki, et al. Structural-Functional Correlations between Unique N-terminal Region and C-terminal Conserved Motif in Short-chain cis-Prenyltransferase from Tomato. ChemBioChem. 2024
Taro Yanai, Yukino Takahashi, Eri Katsumura, Naoki Sakai, Kohei Takeshita, Riki Imaizumi, Hiroaki Matsuura, Shuntaro Hongo, Toshiyuki Waki, Seiji Takahashi, et al. Structural insights into a bacterial β-glucosidase capable of degrading sesaminol triglucoside to produce sesaminol: Toward the understanding of the aglycone recognition mechanism by the C-terminal lid domain. The Journal of Biochemistry. 2023. 174. 4. 335-344

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MISC (46)：

和氣駿之. フラボノイド生合成を制御するメタボロン. 生物工学会誌. 2023. 101. 9. 493-496
矢内太朗, 高橋由季乃, 坂井直樹, 竹下浩平, 今泉璃城, 松浦滉明, 宮原一真, 和氣駿之, 高橋征司, 山本雅貴, et al. セサミノール配糖体加水分解酵素のX線結晶構造解析によって示されたC末端ドメインの新奇機能. 日本生化学会大会(Web). 2023. 96th
門脇芽以, 和氣駿之, 藤田直樹, 沼野井一輝, 佐藤誠哉, 寺下美穂, 福田敬志, 加藤幹也, 根岸尚志, 内田弘美, et al. Identification and characterization of C-glycosyltransferases from Carthamus tinctorius L. 日本植物生理学会年会(Web). 2023. 64th
KHAN Nadia Nur Shazana Binti Abu Talib, 小島幸治, 山口晴彦, 三上智世, 廣森美樹, 和氣駿之, 宮城ゆき乃, 山下哲, 戸澤譲, 中山亨, et al. Solubilization of natural rubber biosynthetic enzyme complexes from rubber particles of the Para rubber tree (Hevea brasiliensis) by the amphiphilic copolymer treatments. 日本植物生理学会年会(Web). 2023. 64th
和氣駿之. フラボノイド生合成における縁の下の力持ち. 生物工学会誌. 2022. 100. 5. 254-254

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Patents (1)：

EFPタンパク質を発現する大腸菌およびそれを用いたフラボノイド化合物製造方法

Lectures and oral presentations (12)：

A regulatory mechanism of flavonoid biosynthetic enzyme activity via protein-protein interaction
(2023)
Inhibition mechanism of chalcone synthase by CoA-SH
(2022)
Identification and functional characterization of enzyme involved in carthamin biosynthesis in safflower (Carthamus tinctorius L.)
(2021)
フラボノイド生合成に進化的に保存されたカルコン合成酵素の活性制御機構とフラボノイドメタボロン
(日本ビタミン学会第72回大会 2020)
Analysis of molecular mechanism involved in activation of chalcone synthase by means of Enhancer of Flavonoid Production
(2019)

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Professional career (1)：

博士(工学) (Tohoku University)

Work history (1)：

2016/10 - 現在 Tohoku University Graduate School of Engineering Department of Biomolecular Engineering

Awards (2)：

2018/09 - 公益社団法人日本農芸化学会東北支部 2018年度日本農芸化学会東北支部若手奨励賞フラボノイド生合成における多酵素複合体(メタボロン)に関する研究
2016/09 - The JSPCMB Student Award

Association Membership(s) (2)：

JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY , JAPANESE SOCIETY FOR PLANT CELL AND MOLECULAR BIOLOGY

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