Art

J-GLOBAL ID：200902242109711328   Reference number：04A0591885

Cloning and Overexpression of the Exiguobacterium sp. F42 Gene Encoding a New Short Chain Dehydrogenase, Which Catalyzes the Stereoselective Reduction of Ethyl 3-Oxo-3-(2-thienyl)propanoate to Ethyl (S)-3-Hydroxy-3-(2-thienyl)propanoate

エチル(S)-3-ヒドロキシ-3-(2-チエニル)プロパノエイトへのエチル3-オキソ-3-(2-チエニル)プロパノエイトの立体選択的還元の触媒となる新短鎖デヒドロゲナーゼをコードするExiguobacterium属F42遺伝子のクローニングと過剰発現

Author (7)： WADA M (Fukui Prefectural Univ., Fukui, JPN) ,  YOSHIZUMI A (Fukui Prefectural Univ., Fukui, JPN) ,  FURUKAWA Y (Fukui Prefectural Univ., Fukui, JPN) ,  KAWABATA H (Mitsubishi Chemical Group Sci. and Technol. Res. Center, Inc., Yokohama, JPN) ,  UEDA M (Mitsubishi Chemical Group Sci. and Technol. Res. Center, Inc., Yokohama, JPN) ,  TAKAGI H (Fukui Prefectural Univ., Fukui, JPN) ,  NAKAMORI S (Fukui Prefectural Univ., Fukui, JPN)
Material： Bioscience, Biotechnology, and Biochemistry  (Bioscience Biotechnology & Biochemistry)
Volume： 68  Issue： 7  Page： 1481-1488  Publication year： Jul. 23, 2004 
JST Material Number： G0021A  ISSN： 0916-8451  CODEN： BBBIEJ  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *oxidoreductase ,...
Semi thesaurus term： carrier

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JST classification (3)： Enzyme physiology  (EG03052C) ,  Enzyme in general  (EB09010D) ,  Molecular genetics in general  (EC02010J)

Substance index (5)： Serotonin ,  NADPH ,  Serine ,  Tyrosine ,  Lysine

Reference (22)：

• 1) Noyori, R., and Ohkuma, T., Asymmetric catalysis by architectural and functional molecular engineering: practical chemo- and stereoselective hydrogenation of ketones. Angew. Chem. Int. Ed., 40, 40–73 (2001).
• 2) Mimoun, H., Laumer, J. Y. S., Giannini, L., Scopelliti, R., and Floriani, C., Enantioselective reduction of ketones by polymethylhydrosiloxane in the presence of chiral zinc catalysis. J. Am. Chem. Soc., 121, 6158–6166 (1999).
• 3) Devaux-Basseguy, R., Bergel, A., and Comtat, M., Potential applications of NAD(P)-dependent oxidoreductases in synthesis: a survey. Enzyme Microb. Technol., 20, 248–258 (1997).
• 4) Kataoka, M., Kita, K., Wada, M., Yasohara, Y., Hasegawa, J., and Shimizu, S., Novel bioreduction system for the production of chiral alcohols. Appl. Microbiol. Biotechnol., 62, 437–445 (2003).
• 5) Wada, M., Yoshizumi, A., Noda, Y., Kataoka, M., Shimizu, S., Takagi, H., and Nakamori, S., Production of doubly chiral compound, (4R,6R)-4-hydroxy-2,2,6-trimethylcyclohexanone, by two-step enzymatic asymmetric reduction. Appl. Environ. Microbiol., 69, 933–937 (2003).

Terms in the title (8)： 立体 ,  選択的還元 ,  触媒 ,  デヒドロゲナーゼ ,  Exiguobacterium属 ,  遺伝子 ,  クローニング ,  過剰発現