Wada Masaru

ワダマサル | Wada Masaru

Affiliation and department：
Homepage URL (1)： https://www.setsunan.ac.jp/~amblab_k/index.html

Research field (2)： Applied microbiology , Applied biochemistry

Research keywords (20)： PLP酵素 , ヒドロキシアスパラギン酸 , 不斉還元 , デヒドラターゼ , 微生物代謝 , スクリーニング , デヒドラターゼ反応 , 酵素的不斉還元 , 新奇酵素 , 立体選択性 , セリンラセマーゼ , アゼチジン-2-カルボン酸 , 出芽酵母 , 転写調節因子 , PCR , 異常タンパク質 , ケノデオキシコール酸 , セリンアセチルトランスフェラーゼ , メチオニン , L-アゼチジン-2-カルボン酸

Research theme for competitive and other funds (10)：

2020 - 2023 Elucidation of sulfur-assimilation mechanism in bifidobacteria
2019 - 2022 Metabolic analysis of an Escherichia coli mutant defective in respiratory chain enzymes and its application to the production of 1,3-butanediol and gamma-aminobutyric acid.
2011 - 2011 グルタミン酸生産菌の代謝工学の新展開:オキサロ酢酸過剰生産型代謝への改変
2007 - 2008 Structural analysis of β-hydroxyaspartate dehydratase
2005 - 2006 新規β-ヒドロキシアスパラギン酸変換酵素の探索

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Papers (60)：

Akinobu Shinmori, Zhen Guo, Tomoya Maeda, Satoru Fukiya, Masaru Wada, Atsushi Yokota. Contributions of the anaplerotic reaction enzymes pyruvate carboxylase and phosphoenolpyruvate carboxylase to l-lysine production in Corynebacterium glutamicum. Journal of Bioscience and Bioengineering. 2024. 138. 3. 225-231
Masaru Wada, Satoru Fukiya, Azusa Suzuki, Nanae Matsumoto, Miki Matsuo, Atsushi Yokota. Methionine utilization by bifidobacteria: possible existence of a reverse transsulfuration pathway. Bioscience of microbiota, food and health. 2021. 40. 1. 80-83
Naoya Kataoka, Alisa S Vangnai, Thunyarat Pongtharangkul, Toshiharu Yakushi, Masaru Wada, Atsushi Yokota, Kazunobu Matsushita. Engineering of Corynebacterium glutamicum as a prototrophic pyruvate-producing strain: Characterization of a ramA-deficient mutant and its application for metabolic engineering. Bioscience, biotechnology, and biochemistry. 2019. 83. 2. 372-380
Hiroyuki Nagano, Kana Shibano, Yu Matsumoto, Atsushi Yokota, Masaru Wada. Isolation and amino acid sequence of a dehydratase acting on d-erythro-3-hydroxyaspartate from Pseudomonas sp. N99, and its application in the production of optically active 3-hydroxyaspartate. Bioscience, biotechnology, and biochemistry. 2017. 81. 6. 1156-1164
Soya Maeda, Kumiko Shimizu, Chie Kihira, Yuki Iwabu, Ryuichi Kato, Makoto Sugimoto, Satoru Fukiya, Masaru Wada, Atsushi Yokota. Pyruvate dehydrogenase complex regulator (PdhR) gene deletion boosts glucose metabolism in Escherichia coli under oxygen-limited culture conditions. Journal of bioscience and bioengineering. 2017. 123. 4. 437-443

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MISC (162)：

新森彰信, GUO Zhen, 吹谷智, 和田大, 横田篤. Comparison of contributions of pyruvate carboxylase and phosphoenolpyruvate carboxylase reactions on lysine production by Corynebacterium glutamicum. 日本農芸化学会大会講演要旨集(Web). 2021. 2021
大塚萌音, GUO Zhen, 佐藤桃梨, 愛甲徹, 柳瀬真紀, 吹谷智, 和田大, 横田篤. Elucidation of the mechanism of enhanced lysine production by mutation in citrate synthase gene leading to its reduced activity in Corynebacterium glutamicum. 日本農芸化学会大会講演要旨集(Web). 2021. 2021
宮森那知, 松本菜々恵, 吹谷智, 横田篤, 和田大. Identification of enzyme genes involved in reverse transsulfuration pathway in Bifidobacterium longum 105-A using gene-knockout mutants. 日本農芸化学会大会講演要旨集(Web). 2021. 2021
宮森那知, 松本菜々恵, 吹谷智, 横田篤, 和田大. Identification and functional verification of enzyme genes constituting reverse transsulfuration pathway in Bifidobacterium longum 105-A. 日本乳酸菌学会誌. 2021. 32. 2
新森彰信, GUO Zhen, 和田大, 横田篤. ピルビン酸カルボキシラーゼ遺伝子の欠失がCorynebacterium glutamicumのリジン生産に与える影響. 日本生物工学会大会講演要旨集. 2019. 71st

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Patents (10)：

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Professional career (1)：

博士(農学) (京都大学)

Work history (2)：

2020/04 - 現在 Setsunan University
2011 - 北海道大学 (連合)農学研究科(研究院) 准教授

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