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J-GLOBAL ID：201802253902832362   Reference number：18A0458882

Enhanced Azidolysis by the Formation of Stable Ser-His Catalytic Dyad in a Glycoside Hydrolase Family 10 Xylanase Mutant

糖加水分解酵素ファミリー10キシラナーゼ変異体における安定なSer-His触媒ダイアドの形成によって増強されたアジド分解

Author (6)： Suzuki Ryuichiro (Department of Biological Production, Akita Prefectural University) ,  Suzuki Ryuichiro (Department of Material and Biological Chemistry, Faculty of Science, Yamagata University) ,  Fujimoto Zui (Advanced Analysis Center, National Agriculture and Food Research Organization (NARO)) ,  Kaneko Satoshi (Department of Subtropical Biochemistry and Biotechnology, Faculty of Agriculture, University of the Ryukyus) ,  Hasegawa Tsunemi (Department of Material and Biological Chemistry, Faculty of Science, Yamagata University) ,  Kuno Atsushi (Biotechnology Research Institute for Drug Discovery (BRD), National Institute of Advanced Industrial Science and Technology (AIST))
Material： Journal of Applied Glycoscience  (応用糖質科学)
Volume： 65  Issue： 1  Page： 1-8(J-STAGE)  Publication year： 2018 
JST Material Number： F0614A  ISSN： 1344-7882  CODEN： JAGLFX  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *decomposition ,...
Semi thesaurus term： Streptomyces ,...

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Author keywords (5)： retaining GH10 β-xylanase ,  chemical rescue ,  switching enzyme with azide ,  histidine catalyzed glycosidase ,  rational protein design

JST classification (1)： Applications of enzymes  (EB09100B)

Reference (38)：

• 1) B. Henrissat: A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J., 280, 309-316 (1991).
• 2) B.L. Cantarel, P.M. Coutinho, C. Rancurel, T. Bernard, V. Lombard, and B. Henrissat: The carbohydrate-active enzymes database (CAZy): An expert resource for glycogenomics. Nucleic Acids Res., 37, D233-D238 (2009).
• 3) S. Kaneko, H. Ichinose, Z. Fujimoto, A. Kuno, K. Yura, M. Go, H. Mizuno, I. Kusakabe, and H. Kobayashi: Structure and function of a family 10 beta-xylanase chimera of Streptomyces olivaceoviridis E-86 FXYN and Cellulomonas fimi Cex. J. Biol. Chem., 279, 26619-26626 (2004).
• 4) S. Kaneko, S. Ito, Z. Fujimoto, A. Kuno, H. Ichinose, S. Iwamatsu, and T. Hasegawa: Importance of Interactions of the α-Helices in the Catalytic Domain N- and C- terminals of the Family 10 Xylanase from Streptomyces olivaceoviridis E-86 to the Stability of the Enzyme. J. Appl. Glycosci., 56, 165-171 (2009).
• 5) S. Kaneko, H. Ichinose, Z. Fujimoto, S. Iwamatsu, A. Kuno, and T. Hasegawa: Substrate recognition of a family 10 Xylanase from Streptomyces olivaceoviridis E-86: A study by site-directed mutagenesis to make an hindrance around the entrance toward the substrate-binding cleft. J. Appl. Glycosci., 56, 173-179 (2009).

Terms in the title (11)： 糖加水分解酵素 ,  ファミリー ,  キシラナーゼ ,  変異体 ,  SER ,  HIS ,  触媒 ,  ダイアド ,  増強 ,  アジド ,  分解