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Characterization of Heme-Coordinating Histidyl Residues of an Engineered Six-Coordinated Myoglobin Mutant Based on the Reactivity with Diethylpyrocarbonate, Mass Spectrometry, and Electron Paramagnetic Resonance Spectroscopy
About NAKANISHI Nobuyuki
About Dep. of Molecular Sci. and Material Engineering, Graduate School of Sci. and Technol.
About TAKEUCHI Fusako
About Dep. of Chemistry, Graduate School of Sci., Kobe Univ.
About TAKEUCHI Fusako
About Div. of Sci. of Biological Supramolecular Systems, Graduate School of Integrated Sci., Yokohama City Univ.
About PARK Sam-yong
About Div. of Sci. of Biological Supramolecular Systems, Graduate School of Integrated Sci., Yokohama City Univ.
About HORI Hiroshi
About Div. of Bioengineering, Dep. of Mechanical Sci. and Bioengineering, Graduate School of Engineering Sci., Osaka Univ.
About KIYOTA Kohei
About Div. of Analytical Chemistry, Graduate School of Pharmaceutical Sciences, Osaka Univ.
About UNO Tadayuki
About Div. of Analytical Chemistry, Graduate School of Pharmaceutical Sciences, Osaka Univ.
About TSUBAKI Motonari
About Dep. of Chemistry, Graduate School of Sci., Kobe Univ.
About TSUBAKI Motonari
About CREST, JST
Material：
About Journal of Bioscience and Bioengineering
Volume： 105 Issue： 6 Page： 604-613 (J-STAGE) Publication year： 2008
JST Material Number： G0535B ISSN： 1389-1723 CODEN： JBBIF6 Document type： Article
Article type： 原著論文 Country of issue： Japan (JPN) Language： ENGLISH (EN)
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About myoglobin
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Keywords indexed to the article.
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About electron paramagnetic resonance
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About Molecular structure
Chemical Substance indexed to the Article.
About Ascorbic Acid
- QIN, J. ^1H NMR study of the solution molecular and electronic strucutre of engineered distal myoglobin His^<64>(E7) Val/Val^<68>(E11) His double mutant. Coordination of His^<64>(E11) at the sixth position in both low-spin and high-spin states. J. Biol. Chem. 1994, 269, 1083-1090
- DOU, Y. Alteration of axial coordination by protein engineering in myoglobin. Bisimidazole ligation in the His^<64>→Val/Val^<68>→His double mutant. J. Biol. Chem. 1995, 270, 15993-16001
- UNO, T. Inversion of axial coordination in myoglobin to create a "proximal" ligand binding pocket. Biochemistry. 2003, 42, 10191-10199
- NAKANISHI, N. Characterization of heme-coordinating histidyl residues of cytochrome b_5 based on the reactivity with diethylpyrocarbonate : a mechanism for the opening of axial himidazole rings. J. Biochem. 2006, 140, 561-571
- TAKEUCHI, F. Ascorbate inhibits the carbethoxylation of two histidyl and one tyrosyl residues indispensable for the transmembrane electron transfer reaction of cytochrome b_<561>. Biochemistry. 2001, 40, 4067-4076
Keywords automatically extracted from the title.
About 反応性
About 質量分析
About 電子常磁性共鳴
About スペクトロスコピー
About 遺伝子操作
About ミオグロビン
About 変異体
About ヘム
About 残基
About 特性づけ
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