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J-GLOBAL ID：200902286630884040   Reference number：08A0682710

Characterization of Heme-Coordinating Histidyl Residues of an Engineered Six-Coordinated Myoglobin Mutant Based on the Reactivity with Diethylpyrocarbonate, Mass Spectrometry, and Electron Paramagnetic Resonance Spectroscopy

ジエチルピロカーボネートとの反応性,質量分析,および電子常磁性共鳴スペクトロスコピーに基づく6つの遺伝子操作ミオグロビン変異体のヘム-配位ヒスチジル残基の特性づけ

Author (9)： NAKANISHI Nobuyuki (Dep. of Molecular Sci. and Material Engineering, Graduate School of Sci. and Technol.) ,  TAKEUCHI Fusako (Dep. of Chemistry, Graduate School of Sci., Kobe Univ.) ,  TAKEUCHI Fusako (Div. of Sci. of Biological Supramolecular Systems, Graduate School of Integrated Sci., Yokohama City Univ.) ,  PARK Sam-yong (Div. of Sci. of Biological Supramolecular Systems, Graduate School of Integrated Sci., Yokohama City Univ.) ,  HORI Hiroshi (Div. of Bioengineering, Dep. of Mechanical Sci. and Bioengineering, Graduate School of Engineering Sci., Osaka Univ.) ,  KIYOTA Kohei (Div. of Analytical Chemistry, Graduate School of Pharmaceutical Sciences, Osaka Univ.) ,  UNO Tadayuki (Div. of Analytical Chemistry, Graduate School of Pharmaceutical Sciences, Osaka Univ.) ,  TSUBAKI Motonari (Dep. of Chemistry, Graduate School of Sci., Kobe Univ.) ,  TSUBAKI Motonari (CREST, JST)
Material： Journal of Bioscience and Bioengineering  (J Biosci Bioeng)
Volume： 105  Issue： 6  Page： 604-613 (J-STAGE)  Publication year： 2008 
JST Material Number： G0535B  ISSN： 1389-1723  CODEN： JBBIF6  Document type： Article
Article type： 原著論文  Country of issue： Japan (JPN)  Language： ENGLISH (EN)

Thesaurus term： *myoglobin ,...
Semi thesaurus term： electron paramagnetic resonance

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JST classification (1)： Molecular structure  (EB03020Y)

Substance index (1)： Ascorbic Acid

Reference (26)：

• QIN, J. ^1H NMR study of the solution molecular and electronic strucutre of engineered distal myoglobin His^<64>(E7) Val/Val^<68>(E11) His double mutant. Coordination of His^<64>(E11) at the sixth position in both low-spin and high-spin states. J. Biol. Chem. 1994, 269, 1083-1090
• DOU, Y. Alteration of axial coordination by protein engineering in myoglobin. Bisimidazole ligation in the His^<64>→Val/Val^<68>→His double mutant. J. Biol. Chem. 1995, 270, 15993-16001
• UNO, T. Inversion of axial coordination in myoglobin to create a "proximal" ligand binding pocket. Biochemistry. 2003, 42, 10191-10199
• NAKANISHI, N. Characterization of heme-coordinating histidyl residues of cytochrome b_5 based on the reactivity with diethylpyrocarbonate : a mechanism for the opening of axial himidazole rings. J. Biochem. 2006, 140, 561-571
• TAKEUCHI, F. Ascorbate inhibits the carbethoxylation of two histidyl and one tyrosyl residues indispensable for the transmembrane electron transfer reaction of cytochrome b_<561>. Biochemistry. 2001, 40, 4067-4076

Terms in the title (9)： 反応性 ,  質量分析 ,  電子常磁性共鳴 ,  スペクトロスコピー ,  遺伝子操作 ,  ミオグロビン ,  変異体 ,  ヘム ,  特性づけ